Department of Pharmacology, College of Pharmacy, University of Duhok, Duhok, Iraq.
Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.
J Med Life. 2023 Jan;16(1):22-34. doi: 10.25122/jml-2022-0215.
Measurements of blood cholinesterase (ChE) activities, including those of erythrocytes (EChE), plasma or serum (PChE), and whole blood (WBChE), are used to assess exposure to ChE-inhibiting pesticides. The purpose of this review was to report normal reference ChE activities in the blood of healthy adult humans, as determined by a modified electrometric method. We performed a systematic review following PRISMA guidelines. Single-group meta-analysis of means of PChE, EChE, and WBChE activities of adult healthy subjects was conducted using the random effects model. The programs used were Open-Meta Analyst and Meta-Essentials Version 1.5. Studies selected for analysis comprised 21, 19, and 4 reports of normal reference/baseline PChE, EChE, and WBChE activities in 690, 635, and 121 healthy adult males and/or females, respectively. The meta-analysis showed normal reference values of the means (effect sizes) with 95% CI of PChE, EChE, and WBChE activities of healthy adult subjects, which were 1.078 (1.015, 1.142), 1.075 (1.024, 1.125) and 1.331 (1.226, 1.436), respectively. By the subgroup analysis, heterogeneity (I2>89%) was considerably reduced in females to 4.4% and 30.1% for PChE and EChE, respectively. Funnel plots indicated no publication bias. However, Egger's regression confirmed the symmetry of the data points for PChE and WBChE activities with a significant effect on EChE. This meta-analysis showed normal reference values of PChE, EChE, and WBChE activities, measured by a modified electrometric method, in healthy adult humans.
测定血液胆碱酯酶（ChE）活性，包括红细胞（EChE）、血浆或血清（PChE）和全血（WBChE）的 ChE 活性，用于评估接触 ChE 抑制性农药的情况。本综述的目的是报告用改良的电测法测定的健康成年人群血液中正常参考 ChE 活性。我们按照 PRISMA 指南进行了系统评价。采用随机效应模型对成人健康受试者的 PChE、EChE 和 WBChE 活性的均值进行单组荟萃分析。使用 Open-Meta 分析器和 Meta-Essentials 版本 1.5 程序进行分析。分析中选择的研究包括 21、19 和 4 项关于 690、635 和 121 名健康成年男性和/或女性的正常参考/基线 PChE、EChE 和 WBChE 活性的报告。荟萃分析显示，健康成年受试者的 PChE、EChE 和 WBChE 活性的正常参考均值（效应大小）及其 95%CI 分别为 1.078（1.015，1.142）、1.075（1.024，1.125）和 1.331（1.226，1.436）。通过亚组分析，女性的异质性（I2>89%）分别显著降低至 4.4%和 30.1%，用于 PChE 和 EChE。漏斗图表明没有发表偏倚。然而，Egger 回归证实 PChE 和 WBChE 活性的数据点具有对称性，对 EChE 有显著影响。本荟萃分析显示，用改良的电测法测定的健康成年人群的 PChE、EChE 和 WBChE 活性具有正常参考值。
