Hsieh Yu-Fang, Lin Kuan-Jiuh
La Morongo Co. Laboratory, Berkeley, California 94720, United States.
Department of Chemistry, National Chung Hsing University, Taichung City 402, Taiwan.
ACS Pharmacol Transl Sci. 2024 Jul 15;7(8):2369-2378. doi: 10.1021/acsptsci.4c00218. eCollection 2024 Aug 9.
Myocardial infarction (MI) is a severe cardiovascular event that can lead to death. Cardiac troponin I (cTnI) is an MI biomarker in the circulation system; however, methods for detecting cTnI protein require substantial time, tedious operations, an expensive reader for translating signals, and a lot of reagents. This study aims to create a cTnI protein test kit with results easily distinguished by color differences, explicitly focusing on the resolution between different concentrations that eyes can discern. These results will aid in creating a commercial, portable, convenient, daily-use rapid-test kit. This study proposes a cTnI biosensor that the naked eye can perceive, performs diagnoses based on pattern color, does not require a reader machine, is easy to operate, and is portable. Our device shortens diagnosis time, has a 0.32-200 ng/mL quantitative analysis range in the human serum matrix, achieves a 0.32 ng/mL limit of detection, and exhibits many advantages compared to a traditional cTnI ELISA plate.
心肌梗死（MI）是一种可能导致死亡的严重心血管事件。心肌肌钙蛋白I（cTnI）是循环系统中的一种心肌梗死生物标志物；然而，检测cTnI蛋白的方法需要大量时间、繁琐的操作、昂贵的信号转换读取器以及大量试剂。本研究旨在创建一种cTnI蛋白检测试剂盒，其结果可通过颜色差异轻松区分，特别关注眼睛能够辨别的不同浓度之间的分辨率。这些结果将有助于创建一种商业化、便携式、方便的日常快速检测试剂盒。本研究提出了一种肉眼可感知的cTnI生物传感器，基于图案颜色进行诊断，无需读取机器，操作简便且便于携带。我们的设备缩短了诊断时间，在人血清基质中的定量分析范围为0.32 - 200 ng/mL，检测限达到0.32 ng/mL，与传统的cTnI酶联免疫吸附测定板相比具有许多优势。
