Bharathiar Cancer Theranostics Research Centre (BCTRC), RUSA 2.0, Bharathiar University, Coimbatore, 641 046, India.
Department of Nanoscience and Technology, Bharathiar University, Coimbatore, 641 046, India.
Mikrochim Acta. 2023 Mar 6;190(4):116. doi: 10.1007/s00604-023-05694-2.
A biomimetic bilayer lipid membrane supported MXene based biosensor is reported for electrochemical hybridization detection of the most prevalent and potential BC biomarker BRCA1. 2D MXene nanosheet-anchored gold nanoparticle-decorated biomimetic bilayer lipid membrane (AuNP@BLM) biosensor is used for the attachment of thiolated single-stranded DNA (HS-ssDNA) targeting hybridization detection. The interaction of biomimetic bilayer lipid membrane with 2D MXene nanosheets is explored in this work for the first time. The synergistic combination of MXene and AuNP@BLM has proven to efficiently improve the detection signal to several folds. The sensor provides hybridization signals only to the complementary DNA (cDNA) sequence with a linearity range 10 zM to 1 µM and LOD of 1 zM without the need of any further amplification. The specificity of the biosensor is validated using non-complementary (ncDNA) and double base mis-match oligonucleotide DNA (dmmDNA) sequences. The sensor successfully distinguishes the signal for different target DNAs with good reproducibility indicated by the RSD value of 4.9%. Hence, we envision that the reported biosensor can be used to construct efficient diagnostic point-of-care tools based on molecular affinity interactions.
一种基于仿生双层脂质膜的 MXene 生物传感器被报道用于电化学杂交检测最常见和潜在的 BRCA1 乳腺癌生物标志物。二维 MXene 纳米片锚定的金纳米粒子修饰的仿生双层脂质膜（AuNP@BLM）生物传感器用于硫醇化单链 DNA（HS-ssDNA）靶向杂交检测的附着。这项工作首次探索了仿生双层脂质膜与二维 MXene 纳米片的相互作用。MXene 和 AuNP@BLM 的协同组合已被证明可有效地将检测信号提高数倍。该传感器仅对互补 DNA（cDNA）序列提供杂交信号，线性范围为 10 zM 至 1 µM，检测限为 1 zM，无需任何进一步的扩增。该生物传感器的特异性通过非互补（ncDNA）和双碱基错配寡核苷酸 DNA（dmmDNA）序列进行验证。该传感器能够成功区分不同靶 DNA 的信号，其重复性良好，RSD 值为 4.9%。因此，我们设想所报道的生物传感器可用于构建基于分子亲和力相互作用的高效即时诊断工具。
