Key Laboratory of Intelligent Computing and Signal Processing of Ministry of Education and Information Materials and Intelligent Sensing Laboratory of Anhui Province, and Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui 230601, China.
BGI-Shenzhen, Shenzhen 518083, China.
Bioinformatics. 2023 Mar 1;39(3). doi: 10.1093/bioinformatics/btad120.
Phage genome annotation plays a key role in the design of phage therapy. To date, there have been various genome annotation tools for phages, but most of these tools focus on mono-functional annotation and have complex operational processes. Accordingly, comprehensive and user-friendly platforms for phage genome annotation are needed.
Here, we propose PhaGAA, an online integrated platform for phage genome annotation and analysis. By incorporating several annotation tools, PhaGAA is constructed to annotate the prophage genome at DNA and protein levels and provide the analytical results. Furthermore, PhaGAA could mine and annotate phage genomes from bacterial genome or metagenome. In summary, PhaGAA will be a useful resource for experimental biologists and help advance the phage synthetic biology in basic and application research.
PhaGAA is freely available at http://phage.xialab.info/.
噬菌体基因组注释在噬菌体治疗的设计中起着关键作用。迄今为止，已经有各种用于噬菌体的基因组注释工具，但这些工具大多侧重于单一功能的注释，并且操作过程复杂。因此，需要一个全面且用户友好的噬菌体基因组注释平台。
在这里，我们提出了 PhaGAA，这是一个用于噬菌体基因组注释和分析的在线集成平台。通过整合多个注释工具，PhaGAA 被构建用于在 DNA 和蛋白质水平上注释噬菌体基因组，并提供分析结果。此外，PhaGAA 还可以从细菌基因组或宏基因组中挖掘和注释噬菌体基因组。总之，PhaGAA 将成为实验生物学家的有用资源，并有助于推进噬菌体合成生物学在基础和应用研究中的发展。
PhaGAA 可免费在 http://phage.xialab.info/ 上获得。
