Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Institute of Medical Photonics, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
J Phys Chem B. 2023 Mar 23;127(11):2381-2394. doi: 10.1021/acs.jpcb.3c00038. Epub 2023 Mar 10.
A lipid droplet (LD) is a dynamic organelle closely associated with cellular functions and energy homeostasis. Dysregulated LD biology underlies an increasing number of human diseases, including metabolic disease, cancer, and neurodegenerative disorder. Commonly used lipid staining and analytical tools have difficulty providing the information regarding LD distribution and composition at the same time. To address this problem, stimulated Raman scattering (SRS) microscopy uses the intrinsic chemical contrast of biomolecules to achieve both direct visualization of LD dynamics and quantitative analysis of LD composition with high molecular selectivity at the subcellular level. Recent developments of Raman tags have further enhanced sensitivity and specificity of SRS imaging without perturbing molecular activity. With these advantages, SRS microscopy has offered great promise for deciphering LD metabolism in single live cells. This article overviews and discusses the latest applications of SRS microscopy as an emerging platform to dissect LD biology in health and disease.
脂滴（LD）是一种与细胞功能和能量稳态密切相关的动态细胞器。LD 生物学的失调是越来越多的人类疾病的基础，包括代谢疾病、癌症和神经退行性疾病。常用的脂质染色和分析工具很难同时提供关于 LD 分布和组成的信息。为了解决这个问题，受激发射拉曼散射（SRS）显微镜利用生物分子的固有化学对比度，实现在亚细胞水平上对 LD 动力学的直接可视化和对 LD 组成的定量分析，同时具有高的分子选择性。拉曼标签的最新发展进一步提高了 SRS 成像的灵敏度和特异性，而不会干扰分子活性。由于这些优势，SRS 显微镜在破译单个活细胞中的 LD 代谢方面具有很大的应用前景。本文综述和讨论了 SRS 显微镜作为一种新兴平台在健康和疾病中解析 LD 生物学的最新应用。
