Kobayashi Misato, Kurimoto Noriaki, Tanino Ryosuke, Shiratsuki Yohei, Okuno Takae, Nakao Mika, Hotta Takamasa, Tsubata Yukari, Nagasaki Makoto, Nishisaka Takashi, Isobe Takeshi
Division of Medical Oncology & Respiratory Medicine, Department of Internal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan.
Division of Pathology, National Hospital Organization Hamada Medical Center, Hamada 697-8511, Japan.
Diagnostics (Basel). 2023 Mar 6;13(5):1003. doi: 10.3390/diagnostics13051003.
Endocytoscopy enables real-time observation of lesions at ultra-magnification. In the gastrointestinal and respiratory fields, endocytoscopic images are similar to hematoxylin-eosin-stained images. This study aimed to compare the nuclear features of pulmonary lesions in endocytoscopic and hematoxylin-eosin-stained images. We performed an endocytoscopy to observe resected specimens of normal lung tissue and lesions. Nuclear features were extracted using ImageJ. We analyzed five nuclear features: nuclear number per area, mean nucleus area, median circularity, coefficient of variation of roundness, and median Voronoi area. We conducted dimensionality reduction analyses for these features, followed by assessments of the inter-observer agreement among two pathologists and two pulmonologists to evaluate endocytoscopic videos. We analyzed the nuclear features of hematoxylin-eosin-stained and endocytoscopic images from 40 and 33 cases, respectively. Endocytoscopic and hematoxylin-eosin-stained images displayed a similar tendency for each feature, despite there being no correlation. Conversely, the dimensionality reduction analyses demonstrated similar distributions of normal lung and malignant clusters in both images, thus differentiating between the clusters. The diagnostic accuracy of the pathologists was 58.3% and 52.8% (κ-value 0.38, fair), and that of the pulmonologists was 50% and 47.2% (κ-value 0.33, fair). The five nuclear features of pulmonary lesions were similar in the endocytoscopic and hematoxylin-eosin-stained images.
内镜下细胞观察术能够以超高倍率实时观察病变。在胃肠道和呼吸道领域，内镜下细胞观察图像类似于苏木精-伊红染色图像。本研究旨在比较内镜下细胞观察图像与苏木精-伊红染色图像中肺病变的细胞核特征。我们对正常肺组织和病变的切除标本进行了内镜下细胞观察。使用ImageJ提取细胞核特征。我们分析了五个细胞核特征：每单位面积的细胞核数量、平均细胞核面积、中位圆形度、圆形度变异系数和中位沃罗诺伊面积。我们对这些特征进行了降维分析，随后评估了两名病理学家和两名肺科医生之间的观察者间一致性，以评估内镜下细胞观察视频。我们分别分析了40例和33例苏木精-伊红染色图像及内镜下细胞观察图像的细胞核特征。尽管不存在相关性，但内镜下细胞观察图像和苏木精-伊红染色图像中各特征均呈现相似趋势。相反，降维分析表明，两种图像中正常肺组织和恶性病变簇的分布相似，从而能够区分这些簇。病理学家的诊断准确率分别为58.3%和52.8%（κ值0.38，一般），肺科医生的诊断准确率分别为50%和47.2%（κ值0.33，一般）。内镜下细胞观察图像和苏木精-伊红染色图像中肺病变的五个细胞核特征相似。
