Department of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia.
Molecules. 2023 Feb 27;28(5):2210. doi: 10.3390/molecules28052210.
The existing study pronounces two newly developed spectrofluorimetric probes for the assay of ambroxol hydrochloride in its authentic and commercial formulations using an aluminum chelating complex and a biogenically mediated and synthesized aluminum oxide nanoparticles (AlONPs) from flower extract. The first probe is based on the formation of an aluminum charge transfer complex. However, the second probe is based on the effect of the unique optical characteristics of AlONPs in the enhancement of fluorescence detection. The biogenically synthesized AlONPs were confirmed using various spectroscopic and microscopic investigations. The fluorescence detections in the two probes were measured at a λ of 260 and 244 and a λ of 460 and 369 nm for the two suggested probes, respectively. The findings showed that the fluorescence intensity (FI) covered linear concentration ranges of 0.1-200 ng mL and 1.0-100 ng mL with a regression of ˃0.999 for AMH-AlONPs-SDS and AMH-Al(NO)-SDS, respectively. The lower detection and quantification limits were evaluated and found to be 0.04 and 0.1 ng mL and 0.7 and 0.1 ng/mL for the abovementioned fluorescence probes, respectively. The two suggested probes were successfully applied for the assay of ambroxol hydrochloride (AMH) with excellent percentage recoveries of 99.65% and 99.85%, respectively. Excipients such as glycerol and benzoic acid used as additives in pharmaceutical preparations, several common cations, and amino acids, as well as sugars, were all found to have no interference with the approach.
现有研究使用铝螯合物和生物合成的氧化铝纳米粒子 (AlONPs) 从花提取物中为盐酸氨溴索的测定开发了两种新的荧光探针。第一种探针基于形成铝电荷转移络合物。然而，第二种探针基于 AlONPs 的独特光学特性在增强荧光检测中的作用。通过各种光谱和显微镜研究证实了生物合成的 AlONPs 的存在。在两个探针中，在 260nm 和 244nm 以及 460nm 和 369nm 处测量了两个建议探针的荧光检测。结果表明，荧光强度 (FI) 涵盖了 0.1-200ng/mL 和 1.0-100ng/mL 的线性浓度范围，对于 AMH-AlONPs-SDS 和 AMH-Al(NO)-SDS，回归系数均大于 0.999。评估并发现上述荧光探针的最低检测限和定量限分别为 0.04ng/mL 和 0.1ng/mL，0.7ng/mL 和 0.1ng/mL。这两种建议的探针成功地应用于盐酸氨溴索 (AMH) 的测定，回收率分别为 99.65%和 99.85%。作为药物制剂添加剂的甘油和苯甲酸等赋形剂、几种常见的阳离子和氨基酸以及糖都不会干扰该方法。
