Phochantachinda Sataporn, Chatchaisak Duangthip, Temviriyanukul Piya, Chansawang Anchana, Pitchakarn Pornsiri, Chantong Boonrat
Prasu-Arthorn Animal Hospital, Faculty of Veterinary Science, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom 73170, Thailand.
Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom 73170, Thailand.
Evid Based Complement Alternat Med. 2021 Sep 7;2021:6405987. doi: 10.1155/2021/6405987. eCollection 2021.
Inhibiting neuroinflammation and modulating neurite outgrowth could be a promising strategy to prevent neurological disorders. (EO) may be a potent agent against them. Although EO extract reportedly has anti-inflammatory properties in macrophages, there is limited knowledge about its neuroprotective activity by suppressing microglia-mediated proinflammatory cytokine production and inducing neurite outgrowth. The present study aimed to elucidate the effect of EO fruit extract on the lipopolysaccharide- (LPS-) induced neuroinflammation using microglial (BV2) and neuroblastoma (Neuro2a) cells. The results demonstrated that, in LPS-treated BV2 cells, EO fruit extract reduced nitric oxide, interleukin-6, and tumor necrotic factor- production. It also enhanced the neurite length of Neuro2a cells, which was linked to the upregulation of TuJ1 and MAP2 expressions. In conclusion, these findings indicate that the ethanolic extract of EO fruits has promising neuroprotective potential to exhibit antineuroinflammation activity and accelerative effect on neurite outgrowth . Therefore, EO fruit extract can be considered a novel herbal medicine candidate for managing neuroinflammatory diseases.
抑制神经炎症和调节神经突生长可能是预防神经系统疾病的一种有前景的策略。（EO）可能是针对这些疾病的一种有效药物。尽管据报道EO提取物在巨噬细胞中具有抗炎特性，但关于其通过抑制小胶质细胞介导的促炎细胞因子产生和诱导神经突生长的神经保护活性的了解有限。本研究旨在使用小胶质细胞（BV2）和成神经细胞瘤（Neuro2a）细胞阐明EO果实提取物对脂多糖（LPS）诱导的神经炎症的影响。结果表明，在LPS处理的BV2细胞中，EO果实提取物减少了一氧化氮、白细胞介素-6和肿瘤坏死因子的产生。它还增加了Neuro2a细胞的神经突长度，这与TuJ1和MAP2表达的上调有关。总之，这些发现表明EO果实的乙醇提取物具有有前景的神经保护潜力，可表现出抗神经炎症活性并对神经突生长有促进作用。因此，EO果实提取物可被认为是治疗神经炎症性疾病的一种新型草药候选物。
