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利用流式细胞术对小鼠T细胞淋巴瘤进行纵向追踪。

Longitudinal tracking of T cell lymphomas in mice using flow cytometry.

作者信息

Kuczynski Elizabeth A, Carnevalli Larissa, Sinclair Charles

机构信息

AstraZeneca, Cambridge CB2 0AA, UK.

AstraZeneca, Cambridge CB2 0AA, UK.

出版信息

STAR Protoc. 2023 Mar 9;4(2):102144. doi: 10.1016/j.xpro.2023.102144.

DOI:10.1016/j.xpro.2023.102144
PMID:36905629
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10024047/
Abstract

T cell hematological cancer has a complex interplay with host immune cells, but the ability to experimentally discriminate transferred cancer cells from host cells by flow cytometry is technically challenging. Here, we present a flow cytometry protocol to evaluate cancer cell and host immune phenotypes following transplant of a T cell lymphoma bearing a congenic marker (CD45.2) into a syngeneic host (CD45.1). We describe steps for isolation of primary immune cells from mice, staining preparation with flow cytometry antibody cocktails, and analysis by flow cytometry. For complete details on the use and execution of this protocol, please refer to Kuczynski et al..

摘要

T细胞血液系统癌症与宿主免疫细胞之间存在复杂的相互作用，但通过流式细胞术在实验上区分转移的癌细胞与宿主细胞的能力在技术上具有挑战性。在这里，我们提出了一种流式细胞术方案，用于评估将携带同基因标记（CD45.2）的T细胞淋巴瘤移植到同基因宿主（CD45.1）后癌细胞和宿主免疫表型。我们描述了从小鼠中分离原代免疫细胞、用流式细胞术抗体混合物进行染色准备以及通过流式细胞术进行分析的步骤。有关该方案的使用和执行的完整详细信息，请参考库钦斯基等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e61/10024047/69fee9d756a6/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e61/10024047/c220fc3545c6/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e61/10024047/7be49026f91b/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e61/10024047/b2550fd579ce/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e61/10024047/69fee9d756a6/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e61/10024047/c220fc3545c6/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e61/10024047/7be49026f91b/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e61/10024047/b2550fd579ce/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e61/10024047/69fee9d756a6/gr3.jpg

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本文引用的文献

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A preclinical model of peripheral T-cell lymphoma GATA3 reveals DNA damage response pathway vulnerability.外周 T 细胞淋巴瘤 GATA3 的临床前模型揭示了 DNA 损伤反应通路的脆弱性。
EMBO Mol Med. 2022 Jun 8;14(6):e15816. doi: 10.15252/emmm.202215816. Epub 2022 May 5.
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PD-1 and ICOS coexpression identifies tumor-reactive CD4+ T cells in human solid tumors.PD-1 和 ICOS 共表达鉴定人类实体瘤中肿瘤反应性 CD4+ T 细胞。
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PI3Kα/δ inhibition promotes anti-tumor immunity through direct enhancement of effector CD8 T-cell activity.
PI3Kα/δ 抑制通过直接增强效应 CD8 T 细胞活性来促进抗肿瘤免疫。
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Combination of dual mTORC1/2 inhibition and immune-checkpoint blockade potentiates anti-tumour immunity.双重mTORC1/2抑制与免疫检查点阻断相结合可增强抗肿瘤免疫力。
Oncoimmunology. 2018 May 7;7(8):e1458810. doi: 10.1080/2162402X.2018.1458810. eCollection 2018.
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Epstein-Barr Virus-Positive CD20- and CD45-Negative Diffuse Large B-Cell Lymphoma: A Diagnostic Challenge.爱泼斯坦-巴尔病毒阳性、CD20和CD45阴性的弥漫性大B细胞淋巴瘤：诊断挑战
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