Sauro Salvatore, Spagnuolo Gianrico, Del Giudice Carmela, Neto Davino M Andrade, Fechine Pierre B A, Chen Xiaohui, Rengo Sandro, Chen Xiaojing, Feitosa Victor P
Dental Biomaterials & Minimally Invasive Dentistry, Departamento de Odontologia, CEU Cardenal Herrera University, Alfara del Patriarca, Valencia, Spain.
Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples "Federico II", 80131 Naples, Italy.
Dent Mater. 2023 Apr;39(4):391-401. doi: 10.1016/j.dental.2023.03.007. Epub 2023 Mar 10.
This study aimed at evaluating the cytotoxicity, chemical and structural properties of experimental fluoride-doped calcium-phosphates as potential remineralising materials for dental applications.
Experimental calcium phosphates were formulated using β-tricalcium phosphate, monocalcium phosphate monohydrate, calcium hydroxide, and different concentrations of calcium/sodium fluoride salts [(5 wt%: VSG5F), (10 wt%: VSG10F), (20 wt%: VSG20F)]. A fluoride-free calcium phosphate (VSG) was used as control. Each tested material was immersed in simulated body fluid (SBF), (24 h, 15 and 30 days) to assess their ability to crystallise into apatite-like. Cumulative fluoride release was assayed up to 45 days. Moreover, each powder was placed into a medium containing human dental pulp stem cells (200 mg/mL) and their cytotoxicity was analysed using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay (24 h, 48 h and 72 h incubation). These latter results were statistically analysed by ANOVA and Tukey's test (α = 0.05).
All the experimental VSG-F materials produced fluoride-containing apatite-like crystals after SBF immersion. VSG20F presented prolonged release of fluoride ions into the storage media (45d). VSG, VSG10F and VSG20F showed a significant cytotoxicity at dilution of 1:1, while at 1:5, only VSG and VSG20F demonstrated a reduction in cell viability. At lower dilutions (1:10, 1:50 and 1:100) all specimens showed no significant toxicity to hDPSCs, but an increase in cell proliferation.
The experimental fluoride-doped calcium-phosphates are biocompatible and possess a clear ability to evoke fluoride-containing apatite-like crystallisation. Hence, they may be promising remineralising materials for dental applications.
本研究旨在评估实验性氟掺杂磷酸钙作为牙科应用潜在再矿化材料的细胞毒性、化学和结构特性。
使用β-磷酸三钙、一水磷酸二氢钙、氢氧化钙和不同浓度的氟化钙/氟化钠盐[(5 wt%: VSG5F)、(10 wt%: VSG10F)、(20 wt%: VSG20F)]配制实验性磷酸钙。使用无氟磷酸钙(VSG)作为对照。将每种测试材料浸入模拟体液(SBF)中(24小时、15天和30天)，以评估它们结晶形成类磷灰石的能力。测定长达45天的累积氟释放量。此外，将每种粉末置于含有人类牙髓干细胞(200 mg/mL)的培养基中，并使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐(MTT)法(孵育24小时、48小时和72小时)分析其细胞毒性。通过方差分析和Tukey检验(α = 0.05)对后一组结果进行统计学分析。
所有实验性VSG-F材料在浸入SBF后均产生含氟类磷灰石晶体。VSG20F向储存介质中释放氟离子的时间延长(45天)。VSG、VSG10F和VSG20F在1:1稀释时表现出显著的细胞毒性，而在1:5稀释时，只有VSG和VSG20F表现出细胞活力降低。在较低稀释度(1:10、1:50和1:100)下，所有标本对人牙髓干细胞均无明显毒性，但细胞增殖增加。
实验性氟掺杂磷酸钙具有生物相容性，并且具有明显的引发含氟类磷灰石结晶的能力。因此，它们可能是有前途的牙科再矿化材料。
