Pontons-Melo Juan Carlos, Balbinot Gabriela de Souza, Sauro Salvatore, Collares Fabrício Mezzomo
Department of Dental Materials, School of Dentistry, Federal University of Rio Grande do Sul. Ramiro Barcelos Street, 2492, Rio Branco, Porto Alegre 90035-003, RS, Brazil.
Dental Biomaterials and Minimally Invasive Dentistry, Department of Dentistry, Cardenal Herrera-CEU University, CEU Universities, C/Santiago Ramón y Cajal, s/n., Alfara del Patriarca, 46115 Valencia, Spain.
J Funct Biomater. 2023 Jun 1;14(6):303. doi: 10.3390/jfb14060303.
The aim of this study was to develop an experimental composite resin with the addition of myristyltrimethylammonium bromide (MYTAB) and α -tricalcium phosphate (α-TCP) as an antibacterial and remineralizing material. Experimental composite resins composed of 75 wt% Bisphenol A-Glycidyl Methacrylate (BisGMA) and 25 wt% Triethylene Glycol Dimethacrylate (TEGDMA) were produced. Some 1 mol% Trimethyl benzoyl-diphenylphosphine oxide (TPO) was used as a photoinitiator, and butylated hydroxytoluene (BTH) was added as a polymerization inhibitor. Silica (1.5 wt%) and barium glass (65 wt%) particles were added as inorganic fillers. For remineralizing and antibacterial effect, α-TCP (10 wt%) and MYTAB (5 wt%) were incorporated into the resin matrix (α-TCP/MYTAB group). A group without the addition of α-TCP/MYTAB was used as a control. Resins were evaluated for their degree of conversion (n = 3) by Fourier Transform Infrared Spectroscopy (FTIR). The flexural strength (n = 5) was assessed based on ISO 4049:2019 requirements. Microhardness was assessed to calculate softening in solvent (n = 3) after ethanol immersion. The mineral deposition (n = 3) was evaluated after immersion in SBF, while cytotoxicity was tested with HaCaT cells (n = 5). Antimicrobial activity (n = 3) was analyzed against The degree of conversion was not influenced by the antibacterial and remineralizing compounds, and all groups reached values > 60%. The α-TCP/MYTAB addition promoted increased softening of polymers after immersion in ethanol and reduced their flexural strength and the viability of cells in vitro. A reduction in viability was observed for the α-TCP/MYTAB group in biofilm formation and planktonic bacteria, with an antibacterial effect > 3log for the developed materials. Higher intensity of phosphate compounds on the sample's surface was detected in the α-TCP/MYTAB group. The addition of α-TCP and MYTAB promoted remineralizing and antibacterial effects on the developed resins and may be a strategy for bioactive composites.
本研究的目的是开发一种添加肉豆蔻基三甲基溴化铵（MYTAB）和α-磷酸三钙（α-TCP）的实验性复合树脂，作为一种抗菌和再矿化材料。制备了由75 wt%双酚A-甲基丙烯酸缩水甘油酯（BisGMA）和25 wt%三乙二醇二甲基丙烯酸酯（TEGDMA）组成的实验性复合树脂。使用约1 mol%的二苯甲酰基二苯基氧化膦（TPO）作为光引发剂，并添加丁基化羟基甲苯（BTH）作为聚合抑制剂。添加二氧化硅（1.5 wt%）和钡玻璃（65 wt%）颗粒作为无机填料。为了实现再矿化和抗菌效果，将α-TCP（10 wt%）和MYTAB（5 wt%）加入树脂中（α-TCP/MYTAB组）。将未添加α-TCP/MYTAB的一组用作对照。通过傅里叶变换红外光谱（FTIR）评估树脂的转化率（n = 3）。根据ISO 4049:2019要求评估弯曲强度（n = 5）。评估显微硬度以计算乙醇浸泡后在溶剂中的软化程度（n = 3）。在模拟体液（SBF）中浸泡后评估矿物质沉积（n = 3），同时用HaCaT细胞测试细胞毒性（n = 5）。分析对[此处原文缺失具体细菌名称]的抗菌活性（n = 3）。转化率不受抗菌和再矿化化合物的影响，所有组的值均>60%。添加α-TCP/MYTAB促进了聚合物在乙醇浸泡后的软化增加，并降低了其弯曲强度和体外细胞活力。观察到α-TCP/MYTAB组在生物膜形成和浮游细菌中的活力降低，所开发材料的抗菌效果>3个对数。在α-TCP/MYTAB组中检测到样品表面磷酸盐化合物的强度更高。添加α-TCP和MYTAB对所开发的树脂具有促进再矿化和抗菌的作用，可能是生物活性复合材料的一种策略。
