基于 SERS 的免疫磁珠用于快速检测 H5N1 流感病毒。
SERS-based immunomagnetic bead for rapid detection of H5N1 influenza virus.
机构信息
Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun China.
Administration for Drug and Instrument Supervision and Inspection of PLAJLSF Beijing China.
出版信息
Influenza Other Respir Viruses. 2023 Mar 9;17(3):e13114. doi: 10.1111/irv.13114. eCollection 2023 Mar.
The surface-enhanced Raman scattering (SERS) has recently drawn attention in the detection of respiratory viruses, but there have been few reports of the direct detection of viruses. In this study, a sandwich immunomagnetic bead SERS was established for the rapid diagnosis of the H5N1 influenza virus. The detection limit was estimated to be 5.0 × 10 TCID/ml. The method showed excellent specificity with no cross-reaction with H1N1, H5N6 or H9N2. The H5N1 influenza virus detection accuracy of the SERS method was 100% in chicken embryos. The results hold great promise for the utilization of SERS as an innovative approach in the diagnosis of influenza virus.
表面增强拉曼散射（SERS）最近在呼吸道病毒检测中引起了关注，但直接检测病毒的报道很少。本研究建立了一种用于快速诊断 H5N1 流感病毒的夹心免疫磁珠 SERS 方法。检测限估计为 5.0×10 TCID/ml。该方法具有优异的特异性，与 H1N1、H5N6 或 H9N2 无交叉反应。SERS 法对鸡胚中 H5N1 流感病毒的检测准确率为 100%。结果表明，SERS 作为一种创新的流感病毒诊断方法具有很大的应用前景。
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