Wang Gang, Zhang Mingming, Zhu Minghua, Zhang Tengfei, Qian Xueqin, Liu Yili, Ma Xinye, Dai Changhao, Wei Dacheng, Zhu Zhaoqin, Sun Juntao, Guo Mingquan
Department of Laboratory Medicine, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200438, China.
Sensors (Basel). 2025 Feb 5;25(3):959. doi: 10.3390/s25030959.
Over the past decade, the large-scale spread of influenza viruses has posed an increasing burden on public health. The effective screening of influenza agents requires a fast, precise, on-site and easy-to-operate method. Unfortunately, current screening methods face challenges in speed and accuracy, especially in complex on-site settings. Here, this work develops a nucleoprotein antibody-modified graphene field-effect transistor (NPAb-GFET) for rapid and highly precise detection of influenza A viruses. The functionalized monoclonal antibodies capture influenza virus nucleoprotein within 100 × 10 s on the sensing surface. Therefore, the developed NPAb-GFET achieves an average response time of 72.1 s when detecting influenza A viruses in clinical samples. Furthermore, the testing of 106 throat swab samples exhibits an accuracy of 99.1%. This finding provides a valuable diagnostic tool for the control of influenza viruses, accelerating the population-wide control of other epidemics.
在过去十年中，流感病毒的大规模传播给公共卫生带来了日益沉重的负担。对流感病原体进行有效筛查需要一种快速、精确、可现场操作且易于操作的方法。不幸的是，目前的筛查方法在速度和准确性方面面临挑战，尤其是在复杂的现场环境中。在此，本研究开发了一种核蛋白抗体修饰的石墨烯场效应晶体管（NPAb-GFET），用于快速、高精度检测甲型流感病毒。功能化的单克隆抗体在传感表面100×10秒内捕获流感病毒核蛋白。因此，所开发的NPAb-GFET在检测临床样本中的甲型流感病毒时平均响应时间为72.1秒。此外，对106份咽拭子样本的检测显示准确率为99.1%。这一发现为控制流感病毒提供了一种有价值的诊断工具，有助于加速对其他流行病的全人群控制。
