Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, Recife, Brazil; Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, Recife, Brazil.
Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, Cidade Universitária, Recife, Brazil.
Int J Biol Macromol. 2020 May 1;150:169-177. doi: 10.1016/j.ijbiomac.2020.02.052. Epub 2020 Feb 7.
In this work, we investigated the antioxidant and immunomodulatory activities of a lignin isolated from Conocarpus erectus leaves. The lignin was characterized by FTIR, H NMR, C NMR and gel permeation chromatography analysis as well as ultraviolet/visible absorption spectra. The lignin was evaluated for total antioxidant activity (TAA), DPPH and ABTS scavenging abilities, and by a lipid peroxidation inhibition assay. Immunomodulatory activity of the lignin (10 μg/mL) on human peripheral blood mononuclear cells (PBMCs) was determined. The C. erectus lignin was found to be of the guaiacyl-syringyl-p-hydroxyphenyl (G-S-H) type, with an average molecular weight of 2709 Da (polydispersity index: 2.1). It showed low TAA (17.92%) and moderate antioxidant activity against DPPH and ABTS (IC: 231.16 and 356.03 μg/mL, respectively). It also inhibited lipid peroxidation by 42.14%. The lignin promoted an increase in mitochondrial ROS levels as well as cytosolic Ca in PBMCs. In addition, it promoted the differentiation and activation of CD8 T lymphocytes, differentiation of CD14 monocytes, and stimulated the release of nitric oxide and cytokines, mainly those linked to a Th1 response. The results showed that the C. erectus lignin may be used in future studies in which the modulation of the immune response is a key factor.
在这项工作中，我们研究了从海檬果叶中分离得到的木质素的抗氧化和免疫调节活性。通过傅里叶变换红外光谱（FTIR）、核磁共振（H NMR 和 C NMR）和凝胶渗透色谱分析以及紫外/可见吸收光谱对木质素进行了表征。评估了木质素的总抗氧化活性（TAA）、DPPH 和 ABTS 清除能力以及脂质过氧化抑制作用。测定了木质素（10μg/mL）对人外周血单核细胞（PBMCs）的免疫调节活性。发现海檬果木质素属于愈创木基-紫丁香基-对羟基苯基（G-S-H）型，平均分子量为 2709Da（多分散指数：2.1）。它表现出低 TAA（17.92%）和中等的 DPPH 和 ABTS 抗氧化活性（IC：231.16 和 356.03μg/mL）。它还能抑制 42.14%的脂质过氧化。木质素促进了 PBMCs 中线粒体 ROS 水平和胞质 Ca2+的增加。此外，它促进了 CD8 T 淋巴细胞的分化和激活、CD14 单核细胞的分化，并刺激一氧化氮和细胞因子的释放，主要是与 Th1 反应相关的细胞因子。结果表明，海檬果木质素可能用于未来的研究中，其中免疫反应的调节是一个关键因素。
