Ren Ranyue, Guo Jiachao, Song Hao, Wei Yong, Luo Chao, Zhang Yayun, Chen Liangxi, Gao Biao, Fu Jijiang, Xiong Wei
Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Department of Pediatric Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Mater Today Bio. 2023 Feb 22;19:100590. doi: 10.1016/j.mtbio.2023.100590. eCollection 2023 Apr.
Implants made of Ti and its alloys are widely utilized in orthopaedic surgeries. However, insufficient osseointegration of the implants often causes complications such as aseptic loosening. Our previous research discovered that disordered titanium dioxide nanorods (TNrs) had satisfactory antibacterial properties and biocompatibility, but TNrs harmed angiogenic differentiation, which might retarded the osseointegration process of the implants. Magnetic nanomaterials have a certain potential in promoting osseointegration, electromagnetic fields within a specific frequency and intensity range can facilitate angiogenic and osteogenic differentiation. Therefore, this study used FeO to endow magnetism to TNrs and explored the regulation effects of Ti, TNrs, and FeO-TNrs under 1 mT 15 Hz sinusoidal electromagnetic field (SEMF) on osteoblastogenesis, osseointegration, angiogenesis, and its mechanism. We discovered that after the addition of SEMF treatment to VR-EPCs cultured on FeO-TNrs, the calcineurin/NFAT signaling pathway was activated, which then reversed the inhibitory effect of FeO-TNrs on angiogenesis. Besides, FeO-TNrs with SEMF enhanced osteogenic differentiation and osseointegration. Therefore, the implant modification mode of FeO-TNrs with the addition of SEMF could more comprehensively promote osseointegration and provided a new idea for the modification of implants.
由钛及其合金制成的植入物在骨科手术中被广泛应用。然而，植入物的骨整合不足常常会引发诸如无菌性松动等并发症。我们之前的研究发现，无序二氧化钛纳米棒（TNrs）具有令人满意的抗菌性能和生物相容性，但TNrs会损害血管生成分化，这可能会阻碍植入物的骨整合过程。磁性纳米材料在促进骨整合方面具有一定潜力，特定频率和强度范围内的电磁场可以促进血管生成和成骨分化。因此，本研究使用FeO赋予TNrs磁性，并探究了在1 mT 15 Hz正弦电磁场（SEMF）作用下，Ti、TNrs和FeO-TNrs对成骨、骨整合、血管生成及其机制的调节作用。我们发现，在对培养于FeO-TNrs上的VR-EPCs进行SEMF处理后，钙调神经磷酸酶/NFAT信号通路被激活，进而逆转了FeO-TNrs对血管生成的抑制作用。此外，SEMF作用下的FeO-TNrs增强了成骨分化和骨整合。因此，添加SEMF的FeO-TNrs植入物改性模式能够更全面地促进骨整合，为植入物改性提供了新思路。
