College of Basic Medicine, Shaanxi University of Chinese Medicine, Xi'an-Xianyang New Economic Zone, Xianyang, 712046, China.
Department of Biomedical Engineering, Fourth Military Medical University, 17 West Changle Road, Xi'an, 710032, China.
Osteoporos Int. 2018 May;29(5):1177-1191. doi: 10.1007/s00198-018-4392-1. Epub 2018 Mar 9.
The effects of exogenous pulsed electromagnetic field (PEMF) stimulation on T1DM-associated osteopathy were investigated in alloxan-treated rabbits. We found that PEMF improved bone architecture, mechanical properties, and porous titanium (pTi) osseointegration by promoting bone anabolism through a canonical Wnt/β-catenin signaling-associated mechanism, and revealed the clinical potential of PEMF stimulation for the treatment of T1DM-associated bone complications.
Type 1 diabetes mellitus (T1DM) is associated with deteriorated bone architecture and impaired osseous healing potential; nonetheless, effective methods for resisting T1DM-associated osteopenia/osteoporosis and promoting bone defect/fracture healing are still lacking. PEMF, as a safe and noninvasive method, have proven to be effective for promoting osteogenesis, whereas the potential effects of PEMF on T1DM osteopathy remain poorly understood.
We herein investigated the effects of PEMF stimulation on bone architecture, mechanical properties, bone turnover, and its potential molecular mechanisms in alloxan-treated diabetic rabbits. We also developed novel nontoxic Ti2448 pTi implants with closer elastic modulus with natural bone and investigated the impacts of PEMF on pTi osseointegration for T1DM bone-defect repair.
The deteriorations of cancellous and cortical bone architecture and tissue-level mechanical strength were attenuated by 8-week PEMF stimulation. PEMF also promoted osseointegration and stimulated more adequate bone ingrowths into the pore spaces of pTi in T1DM long-bone defects. Moreover, T1DM-associated reduction of bone formation was significantly attenuated by PEMF, whereas PEMF exerted no impacts on bone resorption. We also found PEMF-induced activation of osteoblastogenesis-related Wnt/β-catenin signaling in T1DM skeletons, but PEMF did not alter osteoclastogenesis-associated RANKL/RANK signaling gene expression.
We reveal that PEMF improved bone architecture, mechanical properties, and pTi osseointegration by promoting bone anabolism through a canonical Wnt/β-catenin signaling-associated mechanism. This study enriches our basic knowledge for understanding skeletal sensitivity in response to external electromagnetic signals, and also opens new treatment alternatives for T1DM-associated osteopenia/osteoporosis and osseous defects in an easy and highly efficient manner.
研究外源性脉冲电磁场（PEMF）刺激对链脲佐菌素（alloxan）诱导的 1 型糖尿病（T1DM）相关骨病的影响。
我们发现 PEMF 通过经典的 Wnt/β-catenin 信号通路相关机制促进骨合成代谢，改善了骨结构、力学性能和多孔钛（pTi）的骨整合，从而改善了骨结构、力学性能和多孔钛（pTi）的骨整合，揭示了 PEMF 刺激治疗 T1DM 相关骨并发症的临床潜力。
8 周的 PEMF 刺激可减轻松质骨和皮质骨结构以及组织水平力学强度的恶化。PEMF 还促进了 T1DM 长骨缺损中 pTi 的骨整合，并刺激了更多的骨进入 pTi 的孔隙。此外，T1DM 相关的骨形成减少明显被 PEMF 减弱，而 PEMF 对骨吸收没有影响。我们还发现 PEMF 诱导的 T1DM 骨骼中成骨细胞相关 Wnt/β-catenin 信号的激活，但 PEMF 不改变破骨细胞相关 RANKL/RANK 信号基因表达。
我们揭示了 PEMF 通过经典的 Wnt/β-catenin 信号通路相关机制促进骨合成代谢，改善了骨结构、力学性能和 pTi 的骨整合。这项研究丰富了我们对骨骼对外界电磁信号的敏感性的基本认识，为 T1DM 相关骨质疏松/骨量减少和骨缺损的治疗提供了新的选择。
