Jia Qing, Zhang Hui, Zhao Anqi, Qu Lingbo, Xiong Wenlong, Alam Md Asraful, Miao Jixing, Wang Weigao, Li Feihu, Xu Jingliang, Lv Yongkun
School of Chemical Engineering, Zhengzhou University, Zhengzhou, China.
School of Life Sciences, Zhengzhou University, Zhengzhou, China.
Front Bioeng Biotechnol. 2023 Feb 24;11:1156953. doi: 10.3389/fbioe.2023.1156953. eCollection 2023.
D-allulose is a high-value rare sugar with many health benefits. D-allulose market demand increased dramatically after approved as generally recognized as safe (GRAS). The current studies are predominantly focusing on producing D-allulose from either D-glucose or D-fructose, which may compete foods against human. The corn stalk (CS) is one of the main agricultural waste biomass in the worldwide. Bioconversion is one of the promising approach to CS valorization, which is of significance for both food safety and reducing carbon emission. In this study, we tried to explore a non-food based route by integrating CS hydrolysis with D-allulose production. Firstly we developed an efficient whole-cell catalyst to produce D-allulose from D-glucose. Next we hydrolyzed CS and achieved D-allulose production from the CS hydrolysate. Finally we immobilized the whole-cell catalyst by designing a microfluidic device. Process optimization improved D-allulose titer by 8.61 times, reaching 8.78 g/L from CS hydrolysate. With this method, 1 kg CS was finally converted to 48.87 g D-allulose. This study validated the feasibility of valorizing corn stalk by converting it to D-allulose.
D-阿洛酮糖是一种具有多种健康益处的高价值稀有糖。在被批准为公认安全（GRAS）后，D-阿洛酮糖的市场需求急剧增加。目前的研究主要集中在从D-葡萄糖或D-果糖生产D-阿洛酮糖，这可能会与人类争夺食物。玉米秸秆（CS）是全球主要的农业废弃物生物质之一。生物转化是玉米秸秆增值的一种有前景的方法，这对食品安全和减少碳排放都具有重要意义。在本研究中，我们试图通过将玉米秸秆水解与D-阿洛酮糖生产相结合来探索一条非食品基途径。首先，我们开发了一种高效的全细胞催化剂，用于从D-葡萄糖生产D-阿洛酮糖。接下来，我们水解了玉米秸秆，并从玉米秸秆水解物中实现了D-阿洛酮糖的生产。最后，我们通过设计一种微流控装置固定化了全细胞催化剂。工艺优化使D-阿洛酮糖的产量提高了8.61倍，从玉米秸秆水解物中达到了8.78 g/L。通过这种方法，1 kg玉米秸秆最终转化为48.87 g D-阿洛酮糖。本研究验证了通过将玉米秸秆转化为D-阿洛酮糖使其增值的可行性。
