基因组分析揭示了CCK931和YRK005中利用寡糖的碳水化合物活性酶的种类。
Genome analysis revealed a repertoire of oligosaccharide utilizing CAZymes in CCK931 and YRK005.
作者信息
Sharma Neha, Gupta Deepshikha, Park Young-Seo
机构信息
Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120 Republic of Korea.
Department of Plant Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500046 India.
出版信息
Food Sci Biotechnol. 2023 Jan 5;32(4):553-564. doi: 10.1007/s10068-022-01232-7. eCollection 2023 Mar.
UNLABELLED
bacteria are gram-positive, anaerobic, fermentative, and have probiotic potential. This study aimed to compare the genomes of YRK005 and CCK931 isolated from young radish and kimchi, respectively. The genomic size of YRK005 and CCK931 with GC content is 2.36 Mb (45%) and 2.28 Mb (44.67%), respectively. The genome study identified 92 and 83 CAZymes genes, respectively, for YRK005 and CCK931, that are responsible for 26 and 27 glycoside hydrolases (GH) and 21 and 27 glycosyl transferases. Both species have one gene for carbohydrate esterases and three genes for carbohydrate-binding modules. The primary CAZymes found in both species that are involved in oligosaccharide utilization are GH1, GH2, GH30, GH13_30, GH13_31, GH42, GH43, and GH65. The study also details the production pathways for glycogen and folate. Both strains include a unique repertoire of genes, including hypothetical proteins, showing adaptability to diverse ecological niches and evolution over time.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-022-01232-7.
未标记
细菌为革兰氏阳性、厌氧、发酵型，且具有益生菌潜力。本研究旨在比较分别从嫩萝卜和泡菜中分离出的YRK005和CCK931的基因组。YRK005和CCK931的基因组大小及GC含量分别为2.36 Mb（45%）和2.28 Mb（44.67%）。基因组研究分别在YRK005和CCK931中鉴定出92个和83个碳水化合物活性酶（CAZyme）基因，这些基因负责26个和27个糖苷水解酶（GH）以及21个和27个糖基转移酶。这两个物种都有一个碳水化合物酯酶基因和三个碳水化合物结合模块基因。在这两个物种中发现的参与低聚糖利用的主要CAZyme是GH1、GH2、GH30、GH13_30、GH13_31、GH42、GH43和GH65。该研究还详细阐述了糖原和叶酸的产生途径。两种菌株都包含一系列独特的基因，包括假定蛋白，显示出对不同生态位的适应性以及随时间的进化。
补充信息
在线版本包含可在10.1007/s10068 - 022 - 01232 - 7获取的补充材料。
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