Hotchkiss Brain Institute, University of Calgary, Calgary, Canada.
Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada.
Life Sci Alliance. 2023 Mar 13;6(5). doi: 10.26508/lsa.202201806. Print 2023 May.
Single-cell technologies are a method of choice to obtain vast amounts of cell-specific transcriptional information under physiological and diseased states. Myogenic cells are resistant to single-cell RNA sequencing because of their large, multinucleated nature. Here, we report a novel, reliable, and cost-effective method to analyze frozen human skeletal muscle by single-nucleus RNA sequencing. This method yields all expected cell types for human skeletal muscle and works on tissue frozen for long periods of time and with significant pathological changes. Our method is ideal for studying banked samples with the intention of studying human muscle disease.
单细胞技术是在生理和疾病状态下获取大量细胞特异性转录信息的首选方法。成肌细胞因其体积大、多核的特性而难以进行单细胞 RNA 测序。在这里，我们报告了一种新颖、可靠且具有成本效益的方法，可通过单核 RNA 测序分析冷冻的人类骨骼肌。该方法可鉴定出人类骨骼肌的所有预期细胞类型，适用于长时间冷冻和存在明显病理变化的组织。我们的方法非常适合研究储存样本，旨在研究人类肌肉疾病。
