Qin Junjun, Ji Bingzhen, Ma Yijia, Liu Xin, Wang Tian, Liu Guiming, Li Bugao, Wang Guoliang, Gao Pengfei
College of Animal Science, Shanxi Agricultural University, Taigu 030801, China.
Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Heliyon. 2023 Feb 28;9(3):e14020. doi: 10.1016/j.heliyon.2023.e14020. eCollection 2023 Mar.
Viruses are ubiquitous in the gut of animals and play an important role in the ecology of the gut microbiome. The potential effects of these substances on the growth and development of the body are not fully known. Little is known about the effects of breeding environment on pig gut virome. Here, there are 3584 viral operational taxonomic units (vOTUs) longer than 5 kb identified by virus-enriched metagenome sequencing from 25 pig fecal samples. Only a small minority of vOTUs (11.16%) can be classified at the family level, and ∼50% of the genes could be annotated, supporting the concept of pig gut as reservoirs of substantial undescribed viral genetic diversity. The composition of pig gut virome in the six regions may be related to geography. There are only 20 viral clusters (VCs) shared among pig gut virome in six regions of Shanxi Province. These viruses rarely carry antibiotic resistance genes (ARGs). At the same time, they possess abundant auxiliary metabolic genes (AMGs) potentially involved in carbon, sulfur metabolism and cofactor biosynthesis, etc. This study has revealed the unique characteristics and potential function of pig gut DNA virome and established a foundation for the recognition of the viral roles in gut environment.
病毒在动物肠道中普遍存在，并且在肠道微生物群落的生态中发挥着重要作用。这些物质对机体生长发育的潜在影响尚未完全明确。关于养殖环境对猪肠道病毒组的影响知之甚少。在此，通过对25份猪粪便样本进行病毒富集宏基因组测序，鉴定出3584个长度超过5 kb的病毒操作分类单元（vOTU）。只有一小部分vOTU（11.16%）能够在科水平上进行分类，约50%的基因能够被注释，这支持了猪肠道是大量未描述病毒遗传多样性储存库的概念。六个地区猪肠道病毒组的组成可能与地理位置有关。山西省六个地区的猪肠道病毒组中仅共享20个病毒簇（VC）。这些病毒很少携带抗生素抗性基因（ARG）。同时，它们拥有丰富的辅助代谢基因（AMG），可能参与碳、硫代谢以及辅因子生物合成等过程。本研究揭示了猪肠道DNA病毒组的独特特征和潜在功能，为认识病毒在肠道环境中的作用奠定了基础。
