Wang Zhaoyang, Jiang Yajun, Liu Xueting, Lin Weidong, Feng Qianqian, Xin Ting, Guo Xiaoyu, Hou Shaohua, Zhu Hongfei, Jia Hong
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian, Beijing, 100193, China.
Arch Virol. 2020 Oct;165(10):2317-2322. doi: 10.1007/s00705-020-04727-y. Epub 2020 Jul 8.
Canine kobuviruses (CaKoV) have been found in healthy and diarrheic dogs as well as asymptomatic wild carnivores in various countries. In order to investigate the prevalence and evolution of CaKoV in Tangshan, China, 82 dog fecal samples from pet hospitals in Tangshan were subjected to RT-PCR targeting a segment of the 3D gene of CaKoV. Using this method, we identified CaKoV in 14 samples (17.07%, 14/82). Of the CaKoV-positive samples, 78.57% (11/14) and 50% (7/14) were positive for canine parvovirus and canine coronavirus, respectively. The nucleotide sequences of the 14 strains 96.6%-100% identical to each other and 77.6%-99.2% identical to representative sequences from the NCBI GenBank database. We also amplified the 14 VP1 gene sequences and found that they were 93.3%-99.6% identical to each other and 73.3%-97.8% identical to representative sequences from the NCBI GenBank database. Phylogenetic analysis revealed that the 14 CaKoV strains from Tangshan are closely related to those identified in China and Thailand and display less similarity to those found in Africa, the United States, and Europe. Our data suggest that CaKoV circulated in young pet dogs in Tangshan and displays a high co-infection rate with CCoV and CPV. However, the relationship between the three viruses and their roles in the host requires further investigation.
在不同国家，已在健康和腹泻的犬类以及无症状的野生食肉动物中发现了犬杯状病毒（CaKoV）。为了调查中国唐山地区CaKoV的流行情况和进化情况，对来自唐山宠物医院的82份犬粪便样本进行了逆转录聚合酶链反应（RT-PCR），以检测CaKoV 3D基因的一个片段。通过这种方法，我们在14份样本中鉴定出了CaKoV（17.07%，14/82）。在CaKoV阳性样本中，分别有78.57%（11/14）和50%（7/14）的样本同时感染犬细小病毒和犬冠状病毒呈阳性。这14个毒株的核苷酸序列彼此间的相似度为96.6%-100%，与美国国立生物技术信息中心（NCBI）基因库数据库中的代表性序列相似度为77.6%-99.2%。我们还扩增了这14个VP1基因序列，发现它们彼此间的相似度为93.3%-99.6%，与NCBI基因库数据库中的代表性序列相似度为73.3%-97.8%。系统发育分析表明，来自唐山的14个CaKoV毒株与在中国和泰国鉴定出的毒株密切相关，与在非洲、美国和欧洲发现的毒株相似度较低。我们的数据表明，CaKoV在唐山的年轻宠物犬中传播，并且与犬冠状病毒（CCoV）和犬细小病毒（CPV）的共感染率很高。然而，这三种病毒之间的关系及其在宿主中的作用需要进一步研究。
