Heng Yu Chyuan, Menon Nandita, Chen Binbin, Loo Bryan Zong Lin, Wong Garrett Wei Jie, Lim Amber Ching Han, Silvaraju Shaktheeshwari, Kittelmann Sandra
Wilmar International Limited, WIL@NUS Corporate Laboratory, Centre for Translational Medicine, National University of Singapore, Singapore, Singapore.
Int J Syst Evol Microbiol. 2023 Mar;73(3). doi: 10.1099/ijsem.0.005733.
A Gram-stain-positive, rod-shaped, non-spore-forming, catalase-negative, urease-negative, homofermentative and facultatively anaerobic strain, named WILCCON 0076, was isolated from a wild ferment of pieces of a 'Kampung' durian fruit collected on the island of Ubin (Pulau Ubin), Singapore. The durian had fallen to the ground from a durian tree (), on which a group of long-tailed macaques had been observed picking and eating the fruits. Comparative analyses of 16S rRNA gene sequences indicated that WILCCON 0076 potentially represented a novel species within the genus , with the most closely related type strain being DSM 20509 (16S rRNA gene sequence similarity of 97.2 %). Average nucleotide identity and digital DNA-DNA hybridization prediction values were only 86.0% and 18.9 %, respectively. On the basis of the results of a polyphasic approach that included phylogenomic, chemotaxonomic and morphological analyses, we propose a novel species with the name sp. nov. (type strain WILCCON 0076=DSM 114293=LMG 32698).
从新加坡乌敏岛(Pulau Ubin)采集的“甘榜”榴莲果实碎片的野生发酵物中分离出一株革兰氏阳性、杆状、不形成芽孢、过氧化氢酶阴性、脲酶阴性、同型发酵且兼性厌氧的菌株,命名为WILCCON 0076。该榴莲是从一棵榴莲树上掉落到地上的(),有人观察到一群长尾猕猴在这棵树上采摘和食用果实。16S rRNA基因序列的比较分析表明,WILCCON 0076可能代表属内的一个新物种,与之关系最密切的模式菌株是DSM 20509(16S rRNA基因序列相似性为97.2%)。平均核苷酸同一性和数字DNA-DNA杂交预测值分别仅为86.0%和18.9%。基于包括系统基因组学、化学分类学和形态学分析在内的多相方法的结果,我们提出一个新物种,命名为sp. nov.(模式菌株WILCCON 0076 = DSM 114293 = LMG 32698)。