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新种,一种从榴莲果实野生发酵物中分离出的新物种()。

sp. nov., a novel species isolated from the wild ferment of a durian fruit ().

作者信息

Heng Yu Chyuan, Menon Nandita, Chen Binbin, Loo Bryan Zong Lin, Wong Garrett Wei Jie, Lim Amber Ching Han, Silvaraju Shaktheeshwari, Kittelmann Sandra

机构信息

Wilmar International Limited, WIL@NUS Corporate Laboratory, Centre for Translational Medicine, National University of Singapore, Singapore, Singapore.

出版信息

Int J Syst Evol Microbiol. 2023 Mar;73(3). doi: 10.1099/ijsem.0.005733.

Abstract

A Gram-stain-positive, rod-shaped, non-spore-forming, catalase-negative, urease-negative, homofermentative and facultatively anaerobic strain, named WILCCON 0076, was isolated from a wild ferment of pieces of a 'Kampung' durian fruit collected on the island of Ubin (Pulau Ubin), Singapore. The durian had fallen to the ground from a durian tree (), on which a group of long-tailed macaques had been observed picking and eating the fruits. Comparative analyses of 16S rRNA gene sequences indicated that WILCCON 0076 potentially represented a novel species within the genus , with the most closely related type strain being DSM 20509 (16S rRNA gene sequence similarity of 97.2 %). Average nucleotide identity and digital DNA-DNA hybridization prediction values were only 86.0% and 18.9 %, respectively. On the basis of the results of a polyphasic approach that included phylogenomic, chemotaxonomic and morphological analyses, we propose a novel species with the name sp. nov. (type strain WILCCON 0076=DSM 114293=LMG 32698).

摘要

从新加坡乌敏岛(Pulau Ubin)采集的“甘榜”榴莲果实碎片的野生发酵物中分离出一株革兰氏阳性、杆状、不形成芽孢、过氧化氢酶阴性、脲酶阴性、同型发酵且兼性厌氧的菌株,命名为WILCCON 0076。该榴莲是从一棵榴莲树上掉落到地上的(),有人观察到一群长尾猕猴在这棵树上采摘和食用果实。16S rRNA基因序列的比较分析表明,WILCCON 0076可能代表属内的一个新物种,与之关系最密切的模式菌株是DSM 20509(16S rRNA基因序列相似性为97.2%)。平均核苷酸同一性和数字DNA-DNA杂交预测值分别仅为86.0%和18.9%。基于包括系统基因组学、化学分类学和形态学分析在内的多相方法的结果,我们提出一个新物种,命名为sp. nov.(模式菌株WILCCON 0076 = DSM 114293 = LMG 32698)。

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