富含色氨酸的饮食作为研究血清素能肠胰轴的一种新方法。
Tryptophan rich diet as a new approach to study the serotoninergic enteropancreatic axis.
作者信息
Goke B, Richter G, Keim V, Arnold R
机构信息
Dept. of Internal Med. and physiology, Philipps U. of Marburg, FRG
出版信息
Gut. 1987;28 Suppl(Suppl):203-5. doi: 10.1136/gut.28.suppl.203.
The influence of a tryptophan enriched diet (L-tryptophan added as 1% of total diet), fed over 10 days, on the rat duodenum and pancreas was studied by immunohistology, measurements of serotonin and tryptophan tissue concentrations by HPLC, and incubations of pancreatic lobules. Ingestion of a tryptophan enriched diet resulted in increased contents of tryptophan and serotonin in the duodenum that was not accompanied by a significant change of the serotonin cell density. Neither basal nor CCK-stimulated amylase release from isolated pancreatic lobules was altered after tryptophan enriched food. Although serotonin could be extracted from the pancreas, no increase in serotonin concentration was detected after ingestion of the tryptophan diet. A 'serotonin loading' diet may be a useful tool to study the significance of amines produced by gut endocrine cells in respects to enteropancreatic connections.
通过免疫组织学、用高效液相色谱法测量血清素和色氨酸的组织浓度以及胰腺小叶孵育，研究了连续10天喂食富含色氨酸的饮食（添加的L-色氨酸占总饮食的1%）对大鼠十二指肠和胰腺的影响。摄入富含色氨酸的饮食导致十二指肠中色氨酸和血清素含量增加，但血清素细胞密度没有显著变化。喂食富含色氨酸的食物后，分离的胰腺小叶的基础淀粉酶释放和胆囊收缩素刺激的淀粉酶释放均未改变。虽然可以从胰腺中提取出血清素，但摄入色氨酸饮食后未检测到血清素浓度增加。“血清素负荷”饮食可能是一种有用的工具，可用于研究肠道内分泌细胞产生的胺在肠胰连接方面的意义。
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