Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, UK.
Warwick Medical School, University of Warwick, Coventry, UK.
Trends Parasitol. 2023 May;39(5):328-331. doi: 10.1016/j.pt.2023.02.008. Epub 2023 Mar 14.
TrypTag was a 4-year project to tag the N- and C-termini of almost all Trypanosoma brucei proteins with a fluorescent protein and record the subcellular localisation through images and manual annotation. We highlight the new routes to cell biological discovery this transformative resource is enabling for parasitologists and cell biologists.
TrypTag 是一个为期 4 年的项目，旨在对几乎所有的布氏锥虫蛋白的 N 端和 C 端进行荧光蛋白标记，并通过图像和手动注释记录其亚细胞定位。我们强调了这个变革性资源为寄生虫学家和细胞生物学家带来的新的细胞生物学发现途径。
