Pajoum Zeinab, Aliabadi Hooman Aghamirza Moghim, Mohammadi Adibeh, Sadat Zahra, Kashtiaray Amir, Bani Milad Salimi, Shahiri Mohammadali, Mahdavi Mohammad, Eivazzadeh-Keihan Reza, Maleki Ali, Heravi Majid M
Department of Chemistry, School of Physics and Chemistry, Alzahra University, PO Box 1993891176, Vanak, Tehran, Iran.
Advanced Chemical Studies Lab, Department of Chemistry, K. N. Toosi University of Technology, Tehran, Iran.
Heliyon. 2024 Oct 12;10(20):e39073. doi: 10.1016/j.heliyon.2024.e39073. eCollection 2024 Oct 30.
The design and synthesis of biocompatible nanostructures for biomedical applications are considered vital challenges. Herein, a nanobiocomposite based on acacia hydrogel, natural silk fibroin protein, and synthetic protein fibers of polyvinyl alcohol was fabricated and magnetized with iron oxide nanoparticles (FeO MNPs). The structural properties of the hybrid nanobiocomposite were investigated by essential analyses such as Fourier Transform Infrared Spectrometer (FTIR), Field emission scanning electron microscopy (FE-SEM), and X-ray powder diffraction)XRD(analyses, Thermogravimetric and Differential thermogravimetric analysis (TGA-DTG), Vibrating-sample magnetometry (VSM), and Energy Dispersive X-Ray Analysis (EDX). The biological activities and functional properties of the prepared magnetic nanobiocomposite were studied. Results proved that this nanobiocomposite is non-toxic to the healthy HEK293T cell line. In addition, the synthesized nanobiocomposite showed an approximately 22 % reduction in cell viability of BT549 cells after 72 h. All results confirmed the anti-cancer properties of nanobiocomposite against breast cancer cell lines. Therefore, the prepared nanobiocomposite is an excellent material that can use for in-vivo application. Finally, the hyperthermia application was evaluated for this nanobiocomposite. The SAR was measured 93.08 (W/g) at 100 kHz.
设计和合成用于生物医学应用的生物相容性纳米结构被认为是至关重要的挑战。在此，制备了一种基于阿拉伯胶水凝胶、天然丝素蛋白和聚乙烯醇合成蛋白纤维的纳米生物复合材料，并用氧化铁纳米颗粒（FeO MNPs）进行磁化。通过傅里叶变换红外光谱仪（FTIR）、场发射扫描电子显微镜（FE-SEM）、X射线粉末衍射（XRD）分析、热重和微商热重分析（TGA-DTG）、振动样品磁强计（VSM）和能量色散X射线分析（EDX）等基本分析方法研究了杂化纳米生物复合材料的结构性能。研究了制备的磁性纳米生物复合材料的生物活性和功能特性。结果证明，这种纳米生物复合材料对健康的HEK293T细胞系无毒。此外，合成的纳米生物复合材料在72小时后使BT549细胞的细胞活力降低了约22%。所有结果证实了纳米生物复合材料对乳腺癌细胞系的抗癌特性。因此，制备的纳米生物复合材料是一种可用于体内应用的优良材料。最后，对这种纳米生物复合材料的热疗应用进行了评估。在100 kHz时测得的比吸收率（SAR）为93.08（W/g）。
