Zhou Jin-Qi, Zeng Ling-Hui, Li Chen-Tao, He Da-Hong, Zhao Hao-Duo, Xu Yan-Nan, Jin Zi-Tian, Gao Chong
School of Medicine, Institute of Brain and Cognitive Science; Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province, School of Medicine, Hangzhou City University, Hangzhou, Zhejiang Province, China.
Neural Regen Res. 2023 Sep;18(9):1884-1889. doi: 10.4103/1673-5374.367983.
At the level of in vitro drug screening, the development of a phenotypic analysis system with high-content screening at the core provides a strong platform to support high-throughput drug screening. There are few systematic reports on brain organoids, as a new three-dimensional in vitro model, in terms of model stability, key phenotypic fingerprint, and drug screening schemes, and particularly regarding the development of screening strategies for massive numbers of traditional Chinese medicine monomers. This paper reviews the development of brain organoids and the advantages of brain organoids over induced neurons or cells in simulated diseases. The paper also highlights the prospects from model stability, induction criteria of brain organoids, and the screening schemes of brain organoids based on the characteristics of brain organoids and the application and development of a high-content screening system.
在体外药物筛选层面，以高内涵筛选为核心的表型分析系统的发展为高通量药物筛选提供了有力平台。作为一种新型三维体外模型，脑类器官在模型稳定性、关键表型特征以及药物筛选方案方面，尤其是在大量中药单体筛选策略的开发方面，系统性报道较少。本文综述了脑类器官的发展以及脑类器官相较于诱导神经元或细胞在模拟疾病方面的优势。本文还从模型稳定性、脑类器官的诱导标准以及基于脑类器官特征的脑类器官筛选方案和高内涵筛选系统的应用与发展等方面突出了前景。
