Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou, Zhejiang, 310000, China.
Department of Chemistry, Zhejiang University, Hangzhou, Zhejiang, 310000, China.
Adv Healthc Mater. 2023 Jul;12(19):e2300100. doi: 10.1002/adhm.202300100. Epub 2023 Apr 9.
Dentin hypersensitivity (DH) is triggered by external stimuli irking fluid flow through exposed dentinal tubules (DTs). Three commercially available desensitizing agents as control in this study only achieve limited occlusion depths of ≈10 µm in the DTs as well as scarce remineralization of demineralized dentin matrix. Herein, polyelectrolyte-calcium complexes pre-precursor (PCCP) process is proposed for managing DH that demineralized dentin with exposed DTs is rubbed with ultrahighly concentrated polyelectrolyte-calcium suspension (4 g L -5.44 m) followed by phosphate solution (3.25 m), each 10 min, leading to heavy remineralization of demineralized dentin and compact occlusion of the DTs over 200 µm after 1 day of in vitro and in vivo incubation. For the first time, it is demonstrated that the PCCP process relies on the pH-dependent electrostatic attraction between electropositive polyelectrolyte-calcium complexes and electronegative inwalls of DTs comprised of collagen fibrils and hydroxyapatite crystals under alkaline condition. The PCCP process might shed light on a promising dentin desensitizing strategy for DH management via rapid in-depth DT occlusion and remineralization of demineralized dentin.
牙本质敏感症（DH）是由外部刺激引起的，这些刺激会刺激流体通过暴露的牙本质小管（DTs）流动。在这项研究中，三种市售的脱敏剂作为对照，仅能达到约 10 µm 的 DT 闭塞深度，并且对脱矿质牙本质基质的再矿化作用也很少。在此，提出了聚电解质-钙配合物前体（PCCP）处理方法来管理 DH，方法是用超高浓度的聚电解质-钙悬浮液（4 g L -5.44 m）摩擦脱矿质暴露有 DTs 的牙本质，然后用磷酸盐溶液（3.25 m）摩擦 10 分钟，每个溶液 10 分钟，在体外和体内孵育 1 天后，可实现脱矿质牙本质的大量再矿化和 DTs 的紧密闭塞超过 200 µm。首次证明，PCCP 过程依赖于带正电荷的聚电解质-钙配合物与由胶原纤维和羟基磷灰石晶体组成的带负电荷的 DTs 内壁之间在碱性条件下 pH 依赖性静电吸引。PCCP 过程可能为 DH 管理提供一种有前途的牙本质脱敏策略，通过快速深入的 DT 闭塞和脱矿质牙本质的再矿化。
