Sun Bing, Sun Jiao, Zhang Kai, Pang Yanyun, Zhi Cheng, Li Fan, Ye Yangyang, Wang Jinglin, Liu Yongchun, Deng Jiayin, Yang Peng, Zhang Xu
Tianjin Medical University School and Hospital of Stomatology & Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, No. 12 Qixiangtai Road, Heping District, Tianjin 300070, PR China; Tianjin Medical University Institute of Stomatology, No. 12 Qixiangtai Road, Heping District, Tianjin 300070, PR China.
Tianjin Medical University School and Hospital of Stomatology & Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, No. 12 Qixiangtai Road, Heping District, Tianjin 300070, PR China; Tianjin Medical University Institute of Stomatology, No. 12 Qixiangtai Road, Heping District, Tianjin 300070, PR China; Department of Periodontology & Tissue Engineering, School and Hospital of Stomatology, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan, Shandong 250012, China.
Acta Biomater. 2024 Oct 15;188:393-405. doi: 10.1016/j.actbio.2024.08.056. Epub 2024 Sep 6.
Dentin hypersensitivity (DH) manifests as sharp and uncomfortable pain due to the exposure of dentinal tubules (DTs) following the erosion of tooth enamel. Desensitizing agents commonly used in clinical practice have limitations such as limited depth of penetration, slow remineralization and no antimicrobial properties. To alleviate these challenges, our study designed a lactoferrin-derived amyloid nanofilm (PTLF nanofilm) inspired by the saliva-acquired membrane (SAP). The nanofilm utilises Tris(2-carboxyethyl)phosphine (TCEP) to disrupt the disulfide bonds of lactoferrin (LF) under physiological conditions. The PTLF nanofilm modifies surfaces across various substrates and effectively prevents the early and stable adhesion of cariogenic bacteria, such as Streptococcus mutans and Lactobacillus acidophilus. Simultaneously, it adheres rapidly and securely to demineralized dentin surfaces, facilitating in-situ remineralization of HAP through a simple immersion process. This leads to the formation of a remineralized layer resembling natural dentin, with an occlusion depth of dentinal tubules exceeding 80 µm after three days. The in vivo and vitro results confirm that the PTLF nanofilm possesses good biocompatibility and its ability to exert simultaneous antimicrobial effects and dentin remineralization. Accordingly, this innovative bifunctional PTLF amyloid coating offers promising prospects for the management of DH-related conditions. STATEMENT OF SIGNIFICANCE.
牙本质过敏症（DH）表现为由于牙釉质侵蚀后牙本质小管（DTs）暴露而引起的尖锐且不适的疼痛。临床实践中常用的脱敏剂存在局限性，如渗透深度有限、再矿化缓慢且无抗菌性能。为了缓解这些挑战，我们的研究受唾液获得性膜（SAP）启发，设计了一种乳铁蛋白衍生的淀粉样纳米膜（PTLF纳米膜）。该纳米膜利用三（2 - 羧乙基）膦（TCEP）在生理条件下破坏乳铁蛋白（LF）的二硫键。PTLF纳米膜可修饰各种底物表面，并有效防止变形链球菌和嗜酸乳杆菌等致龋菌的早期和稳定黏附。同时，它能快速且牢固地黏附于脱矿牙本质表面，通过简单的浸泡过程促进羟基磷灰石（HAP）的原位再矿化。这导致形成类似于天然牙本质的再矿化层，三天后牙本质小管的封闭深度超过80 µm。体内和体外结果证实PTLF纳米膜具有良好的生物相容性及其同时发挥抗菌作用和牙本质再矿化的能力。因此，这种创新的双功能PTLF淀粉样涂层为牙本质过敏相关病症的治疗提供了广阔前景。重要意义声明。
