Saiga T, Ohbayashi T, Tabuchi K, Midorikawa O
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
In Vitro Cell Dev Biol. 1987 Dec;23(12):850-4. doi: 10.1007/BF02620964.
We developed a method to determine the amount of work performed by cells through cell division in 1.0% agar cultures. There was no correlation between the cloning efficiencies of 1.0 and 0.3% agar cultures. Growth in 1.0% agar cultures correlated well with such malignant properties as tumorigenicity and the invasive and metastatic potentials. Our method revealed that metastatic MC and F cell lines possess different means of taking advantage of energy to proliferate against an environmental pressure from those possessed by nontumorigenic (ME and T-C3H) cell strain/line or nonmetastatic but tumorigenic (L,MR, and magc1) cell lines.
我们开发了一种方法，用于确定细胞在1.0%琼脂培养物中通过细胞分裂所完成的工作量。1.0%琼脂培养物和0.3%琼脂培养物的克隆效率之间没有相关性。1.0%琼脂培养物中的生长与致瘤性、侵袭和转移潜能等恶性特性密切相关。我们的方法表明，转移性MC和F细胞系利用能量增殖以对抗环境压力的方式，与非致瘤性（ME和T-C3H）细胞株/系或非转移性但致瘤性（L、MR和magc1）细胞系所具有的方式不同。
