Larissa Pavanello, Gambrill Benjamin, de Carvalho Rafaela Durrer Parolina, Picolo Mayara Zagui Dal, Cavalli Vanessa, Boaro Letícia Cristina Cidreira, Prokopovich Polina, Cogo-Müller Karina
Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.
School of Pharmacy and Pharmaceutical Science, Cardiff University, Cardiff, United Kingdom.
Dent Mater. 2023 May;39(5):469-477. doi: 10.1016/j.dental.2023.03.005. Epub 2023 Mar 16.
In this study a dentistry nanocomposite with prolonged antibacterial activity using silica nanoparticles (SNPs) loaded with chlorhexidine (CHX) was developed.
SNPs were coated with the Layer-by-Layer technique. Dental composites were prepared with organic matrix of BisGMA/TEGDMA and SNPs with or without CHX (0, 10, 20 or 30% w/w). The physicochemical properties of the developed material were evaluated and agar diffusion method was used to test the antibacterial. In addition, the biofilm inhibitory activity of the composites was evaluated against S. mutans.
SNPs were rounded with diameters about 50 nm, the organic load increased with increasing deposited layers. Material samples with SNPs loaded with CHX (CHX-SNPs) showed the highest values of post-gel volumetric shrinkage, that ranged from 0.3% to 0.81%. Samples containing CHX-SNPs 30% w/w showed the highest values of flexural strength and modulus of elasticity. Only samples containing SNPs-CHX showed growth inhibition against S. mutans, S. mitis and S. gordonii in a concentration-dependent manner. The composites with CHX-SNPs reduced the biofilm formation of S. mutans biofilm at 24 h and 72 h.
The nanoparticle studied acted as fillers and did not interfere with the evaluated physicochemical properties while providing antimicrobial activity against streptococci. Therefore, this initial study is a step forward to the synthesis of experimental composites with improved performance using CHX-SNPs.
在本研究中，开发了一种使用负载洗必泰（CHX）的二氧化硅纳米颗粒（SNP）具有延长抗菌活性的牙科纳米复合材料。
采用层层技术包覆SNP。用BisGMA/TEGDMA有机基质以及含或不含CHX（0、10、20或30%w/w）的SNP制备牙科复合材料。评估所开发材料的物理化学性质，并使用琼脂扩散法测试抗菌性能。此外，评估复合材料对变形链球菌的生物膜抑制活性。
SNP呈圆形，直径约50nm，有机负载量随沉积层数增加而增加。负载CHX的SNP（CHX-SNP）材料样品显示出最高的凝胶后体积收缩值，范围为0.3%至0.81%。含有30%w/w CHX-SNP的样品显示出最高的弯曲强度和弹性模量值。仅含有SNP-CHX的样品对变形链球菌、缓症链球菌和戈氏链球菌有浓度依赖性的生长抑制作用。含CHX-SNP的复合材料在24小时和72小时时减少了变形链球菌生物膜的形成。
所研究的纳米颗粒充当填料，在提供对链球菌的抗菌活性时不干扰所评估的物理化学性质。因此，这项初步研究是朝着使用CHX-SNP合成性能改进的实验性复合材料迈出的一步。
