Department of Botany, Pir Mehr Ali Shah (PMAS)-Arid Agriculture University, Rawalpindi, Pakistan.
Institute of Biology/Plant Physiology, Humboldt-Universität Zü Berlin, Berlin, Germany.
Sci Rep. 2023 Mar 18;13(1):4514. doi: 10.1038/s41598-023-31498-8.
Plants provide humans with more than just food and shelter; they are also a major source of medications. The purpose of this research was to investigate the antioxidant and hypoglycemic potential of green synthesized CeONPs using Mentha royleana leaves extract. The morphological and physicochemical features of CeONPs were evaluated by UV-Visible spectrophotometry, Scanning Electron Microscopy, Energy Dispersive X-rays and Fourier-transform infrared spectrometry, Dynamic light scattering, Atomic Force Microscopy, Zeta Potential. The average size range of synthesized CeONPs diameter between 46 and 56 nm, crystalline in shape, with Polydispersity index value of 0.2 and subatomic particles mean diameter was 4.5-9.1 nm. The antioxidant capability of CeONPs was assessed using DPPH, ABTS, hydrogen peroxide, hydroxyl radical scavenging, and reducing power tests. The hypoglycemic potential of CeONPs was investigated using alpha-amylase, alpha-glucosidase, glucose absorption by yeast cells, and antisucrase. The effective concentrations were 500 and 1000 µg/ml found good in suppressing radical species. To explore the hypoglycemic potential of CeONPs, alpha-amylase, alpha-glucosidase, glucose absorption by yeast cell, and antisucrase assays were performed. Glucose absorb by yeast cells assay was tested for three distinct glucose concentrations: 5 mmol/L, 10 mmol/L, and 25 mmol/L. Green synthesize CeONPs showed a dose-dependent response, higher concentrations of CeONPs imposed a stronger inhibitory impact on the catalytic site of enzymes. This study suggest that CeONPs could possibly binds to the charge carrying species and act as competitive inhibitor which slow down the enzyme substrate reaction and prevents enzymatic degradation. The study's findings were outstanding, which bodes well for future medicinal applications of CeONPs.
植物不仅为人类提供食物和住所，还是药物的主要来源。本研究旨在利用皱叶留兰香（Mentha royleana）叶提取物来研究 CeONPs 的抗氧化和降血糖潜力。CeONPs 的形态和物理化学特性通过紫外可见分光光度法、扫描电子显微镜、能量色散 X 射线和傅里叶变换红外光谱、动态光散射、原子力显微镜和 Zeta 电位进行评估。合成的 CeONPs 的平均粒径范围在 46 至 56nm 之间，呈结晶状，多分散指数值为 0.2，亚原子颗粒平均直径为 4.5-9.1nm。通过 DPPH、ABTS、过氧化氢、羟基自由基清除和还原力试验评估 CeONPs 的抗氧化能力。通过α-淀粉酶、α-葡萄糖苷酶、酵母细胞吸收葡萄糖和蔗糖酶抑制试验研究 CeONPs 的降血糖潜力。发现 500 和 1000µg/ml 的有效浓度可有效抑制自由基。为了探索 CeONPs 的降血糖潜力，进行了α-淀粉酶、α-葡萄糖苷酶、酵母细胞吸收葡萄糖和蔗糖酶抑制试验。酵母细胞吸收葡萄糖试验测试了三个不同的葡萄糖浓度：5mmol/L、10mmol/L 和 25mmol/L。绿色合成的 CeONPs 表现出剂量依赖性反应，较高浓度的 CeONPs 对酶的催化部位产生更强的抑制作用。该研究表明，CeONPs 可能与带电荷的物质结合，并作为竞争性抑制剂，减缓酶-底物反应并防止酶解。研究结果非常出色，为 CeONPs 的未来医学应用带来了良好的前景。
