Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA,
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.
J Avian Med Surg. 2023 Mar;36(4):345-355. doi: 10.1647/21-00076.
Lipid accumulation disorders are common in psittacine birds and can be associated with changes in plasma lipoproteins, most notably low-density lipoprotein (LDL) and high-density lipoprotein (HDL). However, lipoprotein analysis by standard laboratory analyzers or an indirect method, such as the Friedewald formula, has not been validated in parrots. A research colony of 12 Quaker parrots () were used to compare plasma values from the Roche Cobas c501 biochemistry analyzer for total cholesterol, total triglycerides, LDL, and HDL to gel-permeation high-performance liquid chromatography (GP-HPLC). To increase sample size and broaden the analytical range to include dyslipidemic samples, 2 cross-over studies were performed on a 0.3% cholesterol diet and a 20% fat diet. Agreement between methods was assessed by linear mixed models and Bland and Altman plots. The LDL concentrations calculated by the Friedewald formula and alternative formulas, and the effects of triglycerides on the biases, were also evaluated. Forty-five plasma samples were used. The cholesterol diet induced a marked increase in cholesterol and all lipoproteins, whereas the fat diet did not lead to dyslipidemia. Direct and indirect LDL measurements obtained with the clinical analyzer were not in clinical agreement with GP-HPLC, whereas HDL had acceptable agreement for normotriglyceridemic samples. Hypertriglyceridemic plasma samples were found to interfere with lipoprotein measurements. This study found LDL measured by the Roche Cobas c501 biochemistry analyzer and indirect estimations cannot be recommended in the Quaker parrot, and non-HDL cholesterol should be used instead. Lipoprotein panels obtained from hypertriglyceridemic samples should be interpreted with care.
脂类蓄积紊乱在鹦鹉中很常见，并且可能与血浆脂蛋白的变化有关，尤其是低密度脂蛋白（LDL）和高密度脂蛋白（HDL）。然而，标准实验室分析仪或间接方法（如 Friedewald 公式）的脂蛋白分析尚未在鹦鹉中得到验证。使用 12 只和尚鹦鹉的研究群体（）来比较罗氏 Cobas c501 生化分析仪的总胆固醇、总甘油三酯、LDL 和 HDL 的血浆值与凝胶渗透高效液相色谱法（GP-HPLC）。为了增加样本量并扩大分析范围以包括血脂异常样本，在 0.3%胆固醇饮食和 20%脂肪饮食上进行了 2 项交叉研究。通过线性混合模型和 Bland 和 Altman 图评估方法之间的一致性。还评估了 Friedewald 公式和替代公式计算的 LDL 浓度以及甘油三酯对偏差的影响。使用了 45 个血浆样本。胆固醇饮食显著增加了胆固醇和所有脂蛋白，而脂肪饮食并未导致血脂异常。临床分析仪直接和间接测量的 LDL 与 GP-HPLC 不一致，而对于正常甘油三酯样本，HDL 具有可接受的一致性。高甘油三酯血症的血浆样本被发现会干扰脂蛋白的测量。这项研究发现，罗氏 Cobas c501 生化分析仪测量的 LDL 和间接估计值不能在和尚鹦鹉中推荐使用，应改用非 HDL 胆固醇。应谨慎解释从高甘油三酯血症样本中获得的脂蛋白谱。
