Li Wen-Jing, Chen Ju-Hong, Avila Gonzalo A, Ali Muhammad-Yasir, Tian Xin-Yue, Luo Zheng-Yu, Zhang Feng, Shi Shu-Sen, Zhang Jin-Ping
College of Plant Protection, MARA Key Laboratory of Soybean Disease and Pest Control, Jilin Agricultural University, Changchun, China.
MARA-CABI Joint Laboratory for Bio-safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Physiol. 2023 Mar 2;14:1102216. doi: 10.3389/fphys.2023.1102216. eCollection 2023.
The genus includes a number of egg parasitoids that are known to contribute to the control of . The number of progenies, particularly females, is important for the efficient mass rearing of species used in augmentative biological control programs. Cold storage is an important technique for extending the shelf life of natural enemies used in such programs. We assessed how fecundity, sex ratio, lifespan, and the number of hosts parasitized within 24 h were affected by host density for and when offered fresh eggs and how these parameters were affected if adult parasitoids were first placed in cold storage (11°C in the dark) for 19 weeks before being used for propagation. The fecundity were 110.2 and 84.2 offspring emerged at 25°C, for parasitoids not placed in cold storage; among the offspring that emerged, 82.6% and 85.6% were female for and , respectively. If first placed in cold storage, and produced 35.1 and 24.6 offspring per female, respectively, although cold storage significantly extended the shelf life. The survival rates of parasitoids that were placed in cold storage were 90.3% and 81.3% for females, and 3.2% and 0.9% for males of and , respectively. The number of hosts parasitized within 24 h was not shown to be density dependent, but it was significantly lower after cold storage. This information can be used to estimate the likely production for augmented rearing colonies for use in biological control programs.
该属包括一些已知有助于控制[具体对象未明确]的卵寄生蜂。后代数量，尤其是雌性后代数量，对于用于增殖性生物防治计划的物种的高效大规模饲养很重要。冷藏是延长此类计划中使用的天敌保质期的一项重要技术。我们评估了在提供新鲜[具体对象未明确]卵时，寄主密度对[两种未明确的物种]的繁殖力、性别比例、寿命以及24小时内寄生的寄主数量有何影响，以及如果成年寄生蜂在用于繁殖前先在冷藏（黑暗中11°C）中存放19周，这些参数会受到怎样的影响。对于未冷藏的寄生蜂，在25°C时繁殖力分别为110.2和84.2个后代；在孵化出的后代中，[两种未明确的物种]分别有82.6%和85.6%为雌性。如果先冷藏，[两种未明确的物种]每只雌性分别产生35.1和24.6个后代，尽管冷藏显著延长了保质期。冷藏的寄生蜂雌性存活率分别为90.3%和81.3%，[两种未明确的物种]雄性存活率分别为3.2%和0.9%。24小时内寄生的寄主数量未显示出与密度相关，但冷藏后显著降低。这些信息可用于估计用于生物防治计划的增殖饲养群体的可能产量。
