Sangeetha A V, Devi Sheela, Subramanian Anandhalakshmi, Daniel Mary, Anandh Perumal
Laboratory Division, Central Leprosy Teaching and Research Institute, Chengalpattu 604001, Tamil Nadu, India.
Department of Microbiology, Pondicherry Institute of Medical Sciences, Puducherry 605014, India.
J Trop Med. 2023 Mar 8;2023:9910380. doi: 10.1155/2023/9910380. eCollection 2023.
apart from being a colonizer in the genital region is also associated with several other invasive infections in all age groups. With the varied distribution of serotypes across different regions of the world, universal vaccination is also unattainable. However, in India, the knowledge of group B (GBS) genotype distribution is deficient. Thus, this study was initiated to add data on this aspect. . A cross-sectional study was conducted using isolates of group B from all clinical specimens. Along with that, the clinical specimen type and the antibiotic resistance profile of the isolates were correlated with the genotypes recognized through a multiplex PCR assay.
Among the 86 isolates subjected to multiplex PCR for genotype identification, five genotypes were identified with genotype Ib as the predominant one (34.9%), followed by III (20.9%), II (16.3%), Ia (12.7%), and V (11.6%).
The results demonstrated a correlation of types Ib and III with vaginal colonization and type II with urine specimens in the current study. This preliminary study exhibited the distribution of common genotypes and their antibiotic resistance profiles in various GBS isolates. However, multiple studies across the country with larger sample sizes are needed to validate these findings.
除了作为生殖器区域的定植菌外，它还与所有年龄组的其他几种侵袭性感染有关。由于血清型在世界不同地区分布各异，普遍接种疫苗也无法实现。然而，在印度，B组链球菌（GBS）基因型分布的相关知识匮乏。因此，开展了本研究以补充这方面的数据。使用来自所有临床标本的B组分离株进行了一项横断面研究。同时，将临床标本类型和分离株的抗生素耐药谱与通过多重PCR检测识别的基因型进行关联分析。
在86株接受多重PCR进行基因型鉴定的分离株中，鉴定出五种基因型，其中Ib型为主要基因型（34.9%），其次是III型（20.9%）、II型（16.3%）、Ia型（12.7%）和V型（11.6%）。
本研究结果表明，在当前研究中，Ib型和III型与阴道定植相关，II型与尿液标本相关。这项初步研究展示了各种GBS分离株中常见基因型的分布及其抗生素耐药谱。然而，需要在全国范围内开展多项样本量更大的研究来验证这些发现。
