Herrera Santiago, Cordes Erik E
Department of Biological Sciences, Lehigh University, Bethlehem, PA, USA.
Biology Department, Temple University, Philadelphia, PA, USA.
GigaByte. 2023 Mar 16;2023:gigabyte78. doi: 10.46471/gigabyte.78. eCollection 2023.
Like their shallow-water counterparts, cold-water corals create reefs that support highly diverse communities, and these structures are subject to numerous anthropogenic threats. Here, we present the genome assembly of from the southeastern coast of the USA, the first one for a deep-sea scleractinian coral species. We generated PacBio continuous long reads data for an initial assembly and proximity ligation data for scaffolding. The assembly was annotated using evidence from transcripts, proteins, and gene model predictions. This assembly is comparable to high-quality reference genomes from shallow-water scleractinian corals. The assembly comprises 2,858 scaffolds (N50 1.6 Mbp) and has a size of 556.9 Mbp. Approximately 57% of the genome comprises repetitive elements and 34% of coding DNA. We predicted 41,089 genes, including 91.1% of complete metazoan orthologs. This assembly will facilitate investigations into the ecology of this species and the evolution of deep-sea corals.
与浅海同类珊瑚一样，冷水珊瑚形成的珊瑚礁支撑着高度多样化的群落，而这些结构正面临着众多人为威胁。在此，我们展示了来自美国东南海岸的[具体珊瑚物种名称未给出]的基因组组装结果，这是首个深海石珊瑚物种的基因组组装。我们生成了用于初始组装的PacBio连续长读长数据以及用于构建支架的邻近连接数据。该组装利用转录本、蛋白质和基因模型预测的证据进行注释。此组装与浅海石珊瑚的高质量参考基因组相当。该组装包含2858个支架（N50为1.6兆碱基对），大小为556.9兆碱基对。基因组中约57%由重复元件组成，34%为编码DNA。我们预测了41089个基因，其中包括91.1%的完整后生动物直系同源基因。这个组装将有助于对该物种的生态学以及深海珊瑚的进化进行研究。
