非肌肉肌球蛋白II驱动刺丝囊形态发生的关键步骤。

Non-muscle myosin II drives critical steps of nematocyst morphogenesis.

作者信息

Garg Niharika, Štibler Urška Knez, Eismann Björn, Mercker Moritz, Bergheim Bruno Gideon, Linn Anna, Tuchscherer Patrizia, Engel Ulrike, Redl Stefan, Marciniak-Czochra Anna, Holstein Thomas W, Hess Michael W, Özbek Suat

机构信息

University of Heidelberg, Centre for Organismal Studies, Department of Molecular Evolution and Genomics, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany.

Institute for Applied Mathematics, Interdisciplinary Center for Scientific Computing, Heidelberg University, Im Neuenheimer Feld 205, 69120 Heidelberg, Germany.

出版信息

iScience. 2023 Feb 28;26(3):106291. doi: 10.1016/j.isci.2023.106291. eCollection 2023 Mar 17.

DOI:10.1016/j.isci.2023.106291

PMID:36936784

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10014300/

Abstract

Nematocysts are generated by secretion of proteins into a post-Golgi compartment. They consist of a capsule that elongates into a long tube, which is coiled inside the capsule matrix and expelled during its nano-second discharge deployed for prey capture. The driving force for discharge is an extreme osmotic pressure of 150 bar. The complex processes of tube elongation and invagination under these biomechanical constraints have so far been elusive. Here, we show that a non-muscle myosin II homolog (HyNMII) is essential for nematocyst formation in . In early nematocysts, HyNMII assembles to a collar around the neck of the protruding tube. HyNMII then facilitates tube outgrowth by compressing it along the longitudinal axis as evidenced by inhibitor treatment and genetic knockdown. In addition, live imaging of a NOWA::NOWA-GFP transgenic line, which re-defined NOWA as a tube component facilitating invagination, allowed us to analyze the impact of HyNMII on tube maturation.

摘要

刺丝囊是通过蛋白质分泌到高尔基体后区室而产生的。它们由一个胶囊组成，该胶囊延伸成一根长管，长管盘绕在胶囊基质内，并在用于捕获猎物的纳秒级放电过程中被排出。放电的驱动力是150巴的极高渗透压。在这些生物力学限制下，管子伸长和内陷的复杂过程迄今仍不清楚。在这里，我们表明一种非肌肉肌球蛋白II同源物（HyNMII）对于[具体生物名称未给出]中的刺丝囊形成至关重要。在早期刺丝囊中，HyNMII在突出管的颈部周围组装成一个环。然后，如抑制剂处理和基因敲低所证明的，HyNMII通过沿纵轴压缩管子来促进管子生长。此外，NOWA::NOWA-GFP转基因系的实时成像将NOWA重新定义为促进内陷的管成分，这使我们能够分析HyNMII对管成熟的影响。