Castañeda-Corzo Gabriel-Jaime, Infante-Rodríguez Luís-Felipe, Villamil-Poveda Jean-Carlos, Bustillo Jairo, Cid-Arregui Angel, García-Robayo Dabeiba-Adriana
Specialist in Pathology and Oral Surgery, Pontificia Universidad Javeriana, Bogotá, Colombia.
Centro de Investigaciones Odontológicas, Pontificia Universidad Javeriana, Bogotá, Colombia.
Heliyon. 2023 Mar 7;9(3):e14293. doi: 10.1016/j.heliyon.2023.e14293. eCollection 2023 Mar.
To investigate the frequencies and bacterial load of three species of periodontal bacteria in samples from oropharyngeal cancer patients versus healthy individuals.
This is a case-control study based on biopsies collected from tumor tissues obtained from patients with oropharyngeal squamous cell carcinoma between 2016 and 2017 and shed oral mucosal epithelial cells that were collected from controls using the Cepimax® brush, carrying out several brushings towards the posterior third edge of the tongue and the cheek. and detection and absolute quantification was determined through q-PCR. Statistical analysis included a U- test, , Fisher's exact test, odds ratio (OR) and Conditional logistic regression analysis and unconditional regression analysis ( < 0.05).
A total of 48 donors older than 55 years old participated in this study. The population was distributed into 24 patients (cases) and 24 controls. A robust association was established in cases and controls with significance regarding (OR: 15.00) and (OR:11.00). In the comparison between the amount of each bacteria in the groups, showed a higher bacterial load in oropharyngeal cancer patients ( = 0.04). However, multivariate analysis adjusted to the presence of different bacteria and the diverse confounding variables did not reveal significant differences for oropharyngeal cancer association.
and were detected more frequently in the group of patients with cancer. The bivariate analysis of the bacterial load evidenced significant differences for , suggesting that it could be associated with oropharyngeal cancer.
研究三种牙周细菌在口咽癌患者与健康个体样本中的频率及细菌载量。
这是一项病例对照研究，基于2016年至2017年间从口咽鳞状细胞癌患者的肿瘤组织中采集的活检样本，以及使用Cepimax®刷从对照者采集的脱落口腔黏膜上皮细胞，朝着舌后三分之一边缘和脸颊进行多次刷洗。通过q-PCR进行检测和绝对定量。统计分析包括U检验、……、费舍尔精确检验、比值比（OR）以及条件逻辑回归分析和无条件回归分析（P<0.05）。
共有48名55岁以上的捐赠者参与了本研究。人群分为24例患者（病例组）和24例对照。病例组和对照组在……（OR：15.00）和……（OR：11.00）方面建立了显著关联。在比较各组中每种细菌的数量时，口咽癌患者中……显示出更高的细菌载量（P = 0.04）。然而，针对不同细菌的存在和各种混杂变量进行调整的多变量分析未显示口咽癌关联存在显著差异。
……在癌症患者组中检测频率更高。细菌载量的双变量分析表明……存在显著差异，提示其可能与口咽癌有关。
