斑马鱼中的 DNA 甲基化编辑。

DNA methylation editing in zebrafish.

机构信息

Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Sciences, South China Normal University, Guangzhou, P. R. China.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, P. R. China.

出版信息

Epigenetics. 2023 Dec;18(1):2192326. doi: 10.1080/15592294.2023.2192326.

DOI:10.1080/15592294.2023.2192326

PMID:36945831

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10038036/

Abstract

The CRISPR/dCas9-based epigenome editing technique has driven much attention. Fused with a catalytic domain from Dnmt or Tet protein, the CRISPR/dCas9-DnmtCD or -TetCD systems possess the targeted DNA methylation editing ability and have established a series of and disease models. However, no publication has been reported on zebrafish (), an important animal model in biomedicine. The present study demonstrated that CRISPR/dCas9-Dnmt7 and -Tet2 catalytic domain fusions could site-specifically edit genomic DNA methylation in zebrafish and may serve as an efficient toolkit for DNA methylation editing in the zebrafish model.

摘要

基于 CRISPR/dCas9 的表观基因组编辑技术引起了广泛关注。将 CRISPR/dCas9 与 Dnmt 或 Tet 蛋白的催化结构域融合，CRISPR/dCas9-DnmtCD 或 -TetCD 系统具有靶向 DNA 甲基化编辑能力，并建立了一系列和疾病模型。然而，在斑马鱼（）中尚未有报道，斑马鱼是生物医学中重要的动物模型。本研究表明，CRISPR/dCas9-Dnmt7 和 -Tet2 催化结构域融合可以在斑马鱼中特异性编辑基因组 DNA 甲基化，并且可能成为斑马鱼模型中 DNA 甲基化编辑的有效工具包。