Genotypic and Phenotypic Characterization of Some Hypervirulent Clinical Isolates of in a Tertiary Hospital in Hefei, Anhui.

BACKGROUND

is a highly successful pathogen that can cause various infectious diseases, from relatively mild skin infections to life-threatening severe systemic diseases. The widespread pathogenicity of is mainly due to its ability to produce many virulence factors that help destroy various host cells, causing disease. Our primary goal in this study was to explore the genes of highly virulent strains, to identify genes closely associated with high virulence, and to provide ideas for the treatment of infection by highly virulent clinical strains.

RESULTS

This study collected 221 clinical strains from The First Affiliated Hospital Of The University of Science and Technology of China (USTC); their hemolytic abilities were tested. Eight isolates were selected based on their highly hemolytic ability and tested their hemolytic activity again; their phenotypes and gene sequences were also explored. Whole-genome sequencing (WGS) showed six plasmids (pN315, pNE131, pSJH901, pSJH101, SAP106B, and MSSA476), eight antibiotic resistance genes [), and ] and seventy-two virulence related genes. Three highly virulent strains, namely X21111206, 21092239, and 21112607, were found according the infection model. Therefore, we selected 10 representative virulence genes for qRT-PCR: , and . Among them, the expression levels of and , the three isolates, were significantly higher than the positive control NCTC8325.

CONCLUSION

Significant differences appear in the expression of virulence genes in the highly virulent strains, particularly the , It may be that the high expression of gene is the cause of the high virulence of . We can reduce the pathogenicity of by inhibiting the expression of gene, which may provide a strong basis for as a new target for clinical treatment of infection.