Han Xiaoxiao, Ma Yuxuan, Lu Weicheng, Yan Jianfei, Qin Wenpin, He Jiaying, Niu Li-Na, Jiao Kai
The College of Life Science, Northwest University, Xi'an, Shaanxi, China.
State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Key Laboratory of Stomatology, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
Front Bioeng Biotechnol. 2023 Mar 6;11:1138601. doi: 10.3389/fbioe.2023.1138601. eCollection 2023.
Sensory nerves and vessels are critical for skeletal development and regeneration, but crosstalk between neurovascular network and mineralization are not clear. The aim of this study was to explore neurovascular changes and identify bioactive regulators during osteogenesis. osteogenesis model was performed in male rats following Achilles tenotomy. At 3, 6 and 9 weeks after surgery, mineralization, blood vessels, sensory innervation, and bioactive regulators expression were evaluated micro-computed tomography, immunofluorescent staining, histology and reverse transcriptase-polymerase chain reaction analyses. In the process of osteogenesis, the mineral density increased with time, and the locations of minerals, nerves and blood vessels were highly correlated at each time point. The highest density of sensory nerve was observed in the experimental group at the 3rd week, and then gradually decreased with time, but still higher than that in the sham control group. Among many regulatory factors, semaphorin 3A (Sema3A) was highly expressed in experimental model and its expression was temporally sequential and spatially correlated sensory nerve. The present study showes that during osteogenesis, innervation and angiogenesis are highly correlated, and Sema3A is associated with the position and expression of the sensory nerve.
感觉神经和血管对骨骼发育和再生至关重要，但神经血管网络与矿化之间的相互作用尚不清楚。本研究的目的是探讨成骨过程中的神经血管变化并确定生物活性调节因子。在雄性大鼠跟腱切断术后建立成骨模型。在术后3、6和9周，通过微计算机断层扫描、免疫荧光染色、组织学和逆转录聚合酶链反应分析评估矿化、血管、感觉神经支配和生物活性调节因子的表达。在成骨过程中，矿物质密度随时间增加，并且在每个时间点矿物质、神经和血管的位置高度相关。实验组在第3周观察到感觉神经密度最高，然后随时间逐渐降低，但仍高于假手术对照组。在众多调节因子中，信号素3A（Sema3A）在实验模型中高表达，其表达在时间上是连续的，并且在空间上与感觉神经相关。本研究表明，在成骨过程中，神经支配和血管生成高度相关，并且Sema3A与感觉神经的位置和表达相关。
