European Molecular Biology Laboratory, Hamburg, Germany.
Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield, UK.
Methods Mol Biol. 2023;2643:217-232. doi: 10.1007/978-1-0716-3048-8_16.
PCR-based gene targeting enables rapid alteration of the Saccharomyces cerevisiae genome. Here we describe how this method can be applied for directed gene deletions, epitope and fluorescence protein tagging, and conditional gene expression, with a specific focus on peroxisomal proteins.
基于 PCR 的基因靶向使快速改变酿酒酵母基因组成为可能。在这里，我们将描述如何将该方法应用于定向基因缺失、表位和荧光蛋白标记以及条件基因表达，特别关注过氧化物酶体蛋白。
