Jumrani Kanchan, Bhatia Virender Singh, Kataria Sunita, Alamri Saud A, Siddiqui Manzer H, Rastogi Anshu
Division of Plant Physiology, Indian Institute of Soybean Research, Indore 452001, India.
School of Biochemistry, Devi Ahilya Vishwavidyalaya, Indore 452001, India.
Plants (Basel). 2022 Aug 25;11(17):2210. doi: 10.3390/plants11172210.
High temperature is foremost abiotic stress and there are inadequate studies explicating its impact on soybean. In this study, a pot experiment was done in a greenhouse maintained at a day/night temperature of 42/28 °C with a mean temperature of 35 °C to examine the effects of high temperature in soybean plants inoculated with and without arbuscular mycorrhizal fungi (AMF).Various parameters were taken in soybean plants treated with AMF (+) and AMF (-) such as growth analysis, chlorophyll content, canopy temperature, number of stomata, gas exchange, chlorophyll fluorescence, seed yield, and its attributes. It was observed that growth parameters like leaf area, stem height, root length, shoot and root dry biomass were increased in AMF (+) as compared to AMF (-) plants. Chlorophyll content, the number of stomata, photosynthesis rate, stomatal conductance, transpiration rate, and water use efficiency increased in AMF (+) as compared to AMF (-) plants. Chlorophyll fluorescence parameters such as Fv/Fm, Fv/Fo, PhiPSII, fluorescence area, performance index, photochemical quenching, linear electron transport rate, and active reaction centres density of PSII were also found to be enhanced in AMF (+) plants. However, canopy temperature, intercellular CO, Fo/Fm, and non-photochemical quenching were higher in AMF (-) as compared to inoculated plants. An increase in growth and photosynthesis ultimately enhanced the seed yield and its attributes in AMF (+) as compared to AMF (-). Thus, AMF (+) plants have shown much better plant growth, photosynthesis parameters, and seed yield as compared to AMF (-) plants under high temperature. Thus, it is concluded that heat stress-induced damage to the structure and function of the photosynthetic apparatus was alleviated by AMF inoculum. Therefore, AMF can be used as a biofertilizer in alleviating the adverse effects of heat stress in soybean.
高温是首要的非生物胁迫，目前关于其对大豆影响的研究尚不充分。在本研究中，在日间/夜间温度保持为42/28 °C、平均温度为35 °C的温室中进行了盆栽试验，以研究高温对接种和未接种丛枝菌根真菌（AMF）的大豆植株的影响。对接种AMF（+）和未接种AMF（-）的大豆植株测定了各种参数，如生长分析、叶绿素含量、冠层温度、气孔数量、气体交换、叶绿素荧光、种子产量及其相关属性。结果发现，与AMF（-）植株相比，AMF（+）植株的叶面积、茎高、根长、地上部和根部干生物量等生长参数有所增加。与AMF（-）植株相比，AMF（+）植株的叶绿素含量、气孔数量、光合速率、气孔导度、蒸腾速率和水分利用效率均有所提高。AMF（+）植株的叶绿素荧光参数如Fv/Fm、Fv/Fo、PhiPSII、荧光面积、性能指数、光化学猝灭、线性电子传递速率和PSII活性反应中心密度也有所增强。然而，与接种植株相比，AMF（-）植株的冠层温度、细胞间CO₂、Fo/Fm和非光化学猝灭更高。与AMF（-）相比，AMF（+）植株生长和光合作用的增强最终提高了种子产量及其相关属性。因此，与高温下的AMF（-）植株相比，AMF（+）植株在植物生长、光合参数和种子产量方面表现更好。由此得出结论，AMF接种剂减轻了热胁迫对光合器官结构和功能的损害。因此，AMF可作为生物肥料用于减轻大豆热胁迫的不利影响。
