Kato S
Department of Surgery, Nippon Medical School, Tokyo, Japan.
Nihon Geka Gakkai Zasshi. 1987 Aug;88(8):962-9.
The immunologic characteristics of primary cell culture of regenerating mouse liver cells is described. The DNA synthesis of regenerating liver cells isolated after 70% partial hepatectomy was found greater than that without partial hepatectomy. Increased level of stimulated DNA synthesis was noted on 3rd day post hepatectomy, with the highest levels noted on the 4th day. The level of 3H-thymidine incorporation became apparent on day 3 of culture, reaching maximum on day 6 and gradually decreasing there after. Dexamethasone added to the medium increased the attachment rate of liver cells to the culture plate. Insulin and epidermal growth factor added to the medium did not result in any increased DNA synthesis of the liver cells at day 4 of the culture but manifested its effect at day 10. When various cells which were treated with mitomycin C were co-cultured with the liver cells, both normal and regenerating liver cells were suppressed by the addition of normal spleen cells. The degree of suppression was more marked in the culture with regenerating liver cells. When cultured with other immune competent cells, there was no significant difference between the two groups. This suggests that the suppressive activity of the immune competent cells on the cultured regenerating liver cells was not genetically determined.
本文描述了再生小鼠肝细胞原代细胞培养的免疫学特性。发现70%部分肝切除后分离的再生肝细胞的DNA合成大于未进行部分肝切除的细胞。肝切除术后第3天观察到刺激的DNA合成水平增加，第4天达到最高水平。3H-胸腺嘧啶核苷掺入水平在培养第3天开始显现，第6天达到最大值，之后逐渐下降。向培养基中添加地塞米松可提高肝细胞在培养板上的贴壁率。向培养基中添加胰岛素和表皮生长因子在培养第4天未导致肝细胞DNA合成增加，但在第10天表现出其作用。当用丝裂霉素C处理的各种细胞与肝细胞共培养时，添加正常脾细胞会抑制正常和再生肝细胞。在与再生肝细胞的培养中抑制程度更明显。当与其他免疫活性细胞共培养时，两组之间没有显著差异。这表明免疫活性细胞对培养的再生肝细胞的抑制活性不是由基因决定的。
