College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035, People's Republic of China.
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
Sci Rep. 2023 Mar 25;13(1):4897. doi: 10.1038/s41598-023-31647-z.
Diabetes is a common metabolic disorder that has become a major health problem worldwide. In this study, we investigated the role of rutin in attenuating diabetes and preventing diabetes-related colon lesions in mice potentially through regulation of gut microbiota. The rutin from tartary buckwheat as analyzed by HPLC was administered intragastrically to diabetic mice, and then the biochemical parameters, overall community structure and composition of gut microbiota in diabetic mice were assayed. The results showed that rutin lowered serum glucose and improved serum total cholesterol, low-density lipoprotein, high-density lipoprotein, triglyceride concentrations, tumor necrosis factor-α, interleukin-6, and serum insulin in diabetic mice. Notably, rutin obviously alleviated colon lesions in diabetic mice. Moreover, rutin also significantly regulated gut microbiota dysbiosis and enriched beneficial microbiota, such as Akkermansia (p < 0.05). Rutin selectively increased short-chain fatty acid producing bacteria, such as Alistipes (p < 0.05) and Roseburia (p < 0.05), and decreased the abundance of diabetes-related gut microbiota, such as Escherichia (p < 0.05) and Mucispirillum (p < 0.05). Our data suggested that rutin exerted an antidiabetic effect and alleviated colon lesions in diabetic mice possibly by regulating gut microbiota dysbiosis, which might be a potential mechanism through which rutin alleviates diabetes-related symptoms.
糖尿病是一种常见的代谢紊乱疾病，已成为全球主要的健康问题。在这项研究中，我们研究了芦丁通过调节肠道微生物群在减轻糖尿病和预防糖尿病相关结肠病变中的作用。通过高效液相色谱法分析的鞑靼荞麦芦丁通过胃内给药给糖尿病小鼠，然后测定糖尿病小鼠的生化参数、肠道微生物群的整体群落结构和组成。结果表明，芦丁降低了糖尿病小鼠的血清葡萄糖水平，并改善了血清总胆固醇、低密度脂蛋白、高密度脂蛋白、甘油三酯浓度、肿瘤坏死因子-α、白细胞介素-6 和血清胰岛素水平。值得注意的是，芦丁明显缓解了糖尿病小鼠的结肠病变。此外，芦丁还显著调节了肠道微生物群失调，并丰富了有益的微生物群，如阿克曼菌（p < 0.05）。芦丁选择性地增加了短链脂肪酸产生菌，如 Alistipes（p < 0.05）和 Roseburia（p < 0.05），并降低了与糖尿病相关的肠道微生物群的丰度，如 Escherichia（p < 0.05）和 Mucispirillum（p < 0.05）。我们的数据表明，芦丁通过调节肠道微生物群失调发挥抗糖尿病作用并缓解糖尿病小鼠的结肠病变，这可能是芦丁缓解糖尿病相关症状的潜在机制。
