Chen Jin, Tao Jing, Yu Hai-Feng, Ma Cui-Ping, Tan Feng, Wang Xiao-Chun
Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
College of Chemistry and Life Science, Anshan Normal University, Anshan 114016, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Aug 5;296:122648. doi: 10.1016/j.saa.2023.122648. Epub 2023 Mar 21.
The deleterious toxicity of Hg on ecological and biological system makes it crucial for the precise monitoring of Hg. Herein, we prepared a novel "turn-on" chemosensor N'-(4-(methylthio)butan-2-ylidene) rhodamine B hydrazide (denoted as MTRH) by a simple two-step reaction. MTRH exhibited an ultra-low detection limit (LOD) in fluorescence measurement of Hg in pure aqueous media, which was estimated to be 1.3 × 10 mol·L. Moreover, the proposed chemosensor holds the ability of visualizing Hg by the distinct color change of the solution. The corresponding recognition mechanism was investigated by Job's plots, mass spectrometry and DFT calculation analysis. Importantly, the characteristics such as high sensitivity, low cytotoxicity and good biocompatibility of MTRH exhibited in the application of detecting Hg in real water sample and bioimaging of intracellular Hg prove that MTRH is a promising tool to evaluate the levels of Hg in complex biological systems.
汞对生态和生物系统具有有害毒性，因此对汞进行精确监测至关重要。在此，我们通过简单的两步反应制备了一种新型的“开启”化学传感器N'-（4-（甲硫基）丁-2-亚基）罗丹明B酰肼（记为MTRH）。MTRH在纯水性介质中对汞的荧光测量中表现出超低检测限（LOD），估计为1.3×10 mol·L。此外，所提出的化学传感器能够通过溶液明显的颜色变化实现汞的可视化。通过Job曲线、质谱和密度泛函理论（DFT）计算分析研究了相应的识别机制。重要的是，MTRH在实际水样中检测汞以及细胞内汞的生物成像应用中表现出的高灵敏度、低细胞毒性和良好生物相容性等特性证明，MTRH是评估复杂生物系统中汞含量的一种有前途的工具。
