Department of Obstetrics, Jinniu District Maternal and Child Health Hospital, Chengdu, China.
Department of Medical Laboratory, Jinniu District Maternal and Child Health Hospital, Chengdu, China.
Front Immunol. 2023 Mar 9;14:1131870. doi: 10.3389/fimmu.2023.1131870. eCollection 2023.
C15orf48 was recently identified as an inflammatory response-related gene; however there is limited information on its function in tumors. In this study, we aimed to elucidate the function and potential mechanism of action of C15orf48 in cancer.
We evaluated the pan-cancer expression, methylation, and mutation data of C15orf48 to analyze its clinical prognostic value. In addition, we explored the pan-cancer immunological characteristics of C15orf48, especially in thyroid cancer (THCA), by correlation analysis. Additionally, we conducted a THCA subtype analysis of C15orf48 to determine its subtype-specific expression and immunological characteristics. Lastly, we evaluated the effects of C15orf48 knockdown on the THCA cell line, BHT101, by experimentation.
The results of our study revealed that C15orf48 is differentially expressed in different cancer types and that it can serve as an independent prognostic factor for glioma. Additionally, we found that the epigenetic alterations of C15orf48 are highly heterogeneous in several cancers and that its aberrant methylation and copy number variation are associated with poor prognosis in multiple cancers. Immunoassays elucidated that C15orf48 was significantly associated with macrophage immune infiltration and multiple immune checkpoints in THCA, and was a potential biomarker for PTC. In addition, cell experiments showed that the knockdown of C15orf48 could reduce the proliferation, migration, and apoptosis abilities of THCA cells.
The results of this study indicate that C15orf48 is a potential tumor prognostic biomarker and immunotherapy target, and plays an essential role in the proliferation, migration, and apoptosis of THCA cells.
C15orf48 最近被鉴定为与炎症反应相关的基因，但关于其在肿瘤中的功能知之甚少。在这项研究中，我们旨在阐明 C15orf48 在癌症中的功能和潜在作用机制。
我们评估了 C15orf48 的泛癌表达、甲基化和突变数据，以分析其临床预后价值。此外，我们通过相关性分析探讨了 C15orf48 的泛癌免疫特征，特别是在甲状腺癌（THCA）中。此外，我们对 C15orf48 进行了 THCA 亚型分析，以确定其亚型特异性表达和免疫特征。最后，我们通过实验评估了 C15orf48 敲低对 THCA 细胞系 BHT101 的影响。
我们的研究结果表明，C15orf48 在不同癌症类型中表达差异，并且可以作为胶质母细胞瘤的独立预后因素。此外，我们发现 C15orf48 的表观遗传改变在几种癌症中高度异质，其异常甲基化和拷贝数变异与多种癌症的不良预后相关。免疫分析表明，C15orf48 在 THCA 中与巨噬细胞免疫浸润和多个免疫检查点显著相关，是 PTC 的潜在生物标志物。此外，细胞实验表明，C15orf48 的敲低可以降低 THCA 细胞的增殖、迁移和凋亡能力。
本研究结果表明，C15orf48 是一种潜在的肿瘤预后生物标志物和免疫治疗靶点，在 THCA 细胞的增殖、迁移和凋亡中发挥重要作用。
