Kim Jieun, Kim Johyun, Yoon Heewon, Chae Yoon-Jee, Rhew Kiyon, Chang Ji-Eun
College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea.
College of Pharmacy, Dongduk Women's University, Seoul 02748, Republic of Korea.
Curr Issues Mol Biol. 2023 Mar 17;45(3):2474-2490. doi: 10.3390/cimb45030162.
To overcome the limitation of conventional cancer treatments, photodynamic therapy (PDT) has been introduced as another treatment option. PDT provides a non-invasive, non-surgical way with reduced toxicity. To improve the antitumor efficacy of PDT, we synthesized a novel photosensitizer, a 3-substituted methyl pyropheophorbide-a derivative (Photomed). The purpose of the study was to evaluate the antitumor effect of PDT with Photomed comparing with the clinically approved photosensitizers Photofrin and Radachlorin. The cytotoxicity assay against SCC VII cells (murine squamous cell carcinoma) was performed to determine whether Photomed is safe without PDT and whether Photomed is effective against cancer cells with PDT. An in vivo anticancer efficacy study was also performed using SCC VII tumor-bearing mice. The mice were divided into small-tumor and large-tumor groups to identify whether Photomed-induced PDT is effective for not only small tumors but also large tumors. From in vitro and in vivo studies, Photomed was confirmed to be (1) a safe photosensitizer without laser irradiation, (2) the most effective photosensitizer with PDT against cancers compared to Photofrin and Radachlorin and (3) effective with PDT in treating not only small tumors but also large tumors. In conclusion, Photomed may contribute as a novel, potential photosensitizer for use in PDT cancer treatment.
为克服传统癌症治疗方法的局限性，光动力疗法（PDT）已作为另一种治疗选择被引入。PDT提供了一种毒性较低的非侵入性、非手术治疗方式。为提高PDT的抗肿瘤疗效，我们合成了一种新型光敏剂，即3-取代甲基焦脱镁叶绿酸-a衍生物（Photomed）。本研究的目的是评估与临床批准的光敏剂Photofrin和Radachlorin相比，使用Photomed进行PDT的抗肿瘤效果。针对SCC VII细胞（小鼠鳞状细胞癌）进行细胞毒性试验，以确定Photomed在无PDT情况下是否安全以及在有PDT时是否对癌细胞有效。还使用荷SCC VII肿瘤的小鼠进行了体内抗癌疗效研究。将小鼠分为小肿瘤组和大肿瘤组，以确定Photomed诱导的PDT是否不仅对小肿瘤有效，对大肿瘤也有效。通过体外和体内研究证实，Photomed（1）是一种在无激光照射时安全的光敏剂；（2）与Photofrin和Radachlorin相比，是PDT治疗癌症时最有效的光敏剂；（3）PDT不仅对小肿瘤有效，对大肿瘤也有效。总之，Photomed可能作为一种新型、有潜力的光敏剂用于PDT癌症治疗。
