Chen F D, Hendry J H
Br J Radiol. 1986 Apr;59(700):389-95. doi: 10.1259/0007-1285-59-700-389.
A new microcolony technique is described for measuring the survival of colony-forming cells in mouse tail epidermis. The survival curve is characterised by D0 = 2.70 +/- 0.12 Gy. The number of microcolonies per cm2 is similar to the number of macrocolonies after high doses, which shows for the first time that all microcolonies (greater than or equal to 32 cells) in epidermis develop into macrocolonies. At low doses the number of macrocolonies underestimates the number of colony-forming cells because of coalescence of microcolonies to form macrocolonies. This results in a lower apparent sensitivity of macrocolony-forming cells by a factor of about 1.5. About 3% of basal cells in tail epidermis appear to be capable of colony formation.
描述了一种用于测量小鼠尾部表皮中集落形成细胞存活率的新微集落技术。存活曲线的特征为D0 = 2.70±0.12戈瑞。高剂量后每平方厘米微集落的数量与大集落的数量相似，这首次表明表皮中的所有微集落（≥32个细胞）都会发育成大集落。在低剂量时，由于微集落合并形成大集落，大集落的数量会低估集落形成细胞的数量。这导致大集落形成细胞的表观敏感性降低约1.5倍。尾部表皮中约3%的基底细胞似乎能够形成集落。
