Potential Activity of as Antioxidant, Cytotoxic and Antifungal against Some Skin Diseases: Topical Cream Application.

This research evaluated the antifungal effectiveness of ethanol, methanol, ethyl acetate and acetone extracts against the tested pathogenic fungi (, and ). Antioxidant and cytotoxicity effectiveness of extracts against four distinct cell lines were also assessed. Methanol extract of exhibited the highest inhibition zones against as measured by the well diffusion method. A transmission electron micrograph of the treated group of cells with methanolic extract showed mild lysis and vacuolation of the cytoplasmic organelles. In vivo, after induced infection of mice by and treatment with methanolic extract cream, the skin layer emerged with the removal of spherical plastopores. The extract of recorded the highest antioxidant activity using the DPPH (2, 2- diphenyl-1-picrylhydrazyl) scavenging method (IC 28 mg/mL). A cytotoxicity test using a MTT assay showed that the extract had strong cytotoxic activity against the HepG2 cell line (IC 20.56 ± 1.7 μg/mL) and moderate cytotoxic activity against MCF7 and the Hela cell (IC 27.99 ± 2.1 μg/mL). Gas Chromatography/Mass Spectroscopy (GC/MS) results revealed that the effective activity of extract could be linked to a synergistic impact between their prominent composition as alkaloids, phytol, fatty acids hydrocarbons, phenolics and phthalates. extract contains active metabolites that constitute a promising source of antifungal, antioxidant and anti-proliferative compounds for the pharmaceutical drug industry.