Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt.
MARE-Marine and Environmental Sciences Centre, ARNET, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal.
Mar Drugs. 2023 Feb 27;21(3):160. doi: 10.3390/md21030160.
This research evaluated the antifungal effectiveness of ethanol, methanol, ethyl acetate and acetone extracts against the tested pathogenic fungi (, and ). Antioxidant and cytotoxicity effectiveness of extracts against four distinct cell lines were also assessed. Methanol extract of exhibited the highest inhibition zones against as measured by the well diffusion method. A transmission electron micrograph of the treated group of cells with methanolic extract showed mild lysis and vacuolation of the cytoplasmic organelles. In vivo, after induced infection of mice by and treatment with methanolic extract cream, the skin layer emerged with the removal of spherical plastopores. The extract of recorded the highest antioxidant activity using the DPPH (2, 2- diphenyl-1-picrylhydrazyl) scavenging method (IC 28 mg/mL). A cytotoxicity test using a MTT assay showed that the extract had strong cytotoxic activity against the HepG2 cell line (IC 20.56 ± 1.7 μg/mL) and moderate cytotoxic activity against MCF7 and the Hela cell (IC 27.99 ± 2.1 μg/mL). Gas Chromatography/Mass Spectroscopy (GC/MS) results revealed that the effective activity of extract could be linked to a synergistic impact between their prominent composition as alkaloids, phytol, fatty acids hydrocarbons, phenolics and phthalates. extract contains active metabolites that constitute a promising source of antifungal, antioxidant and anti-proliferative compounds for the pharmaceutical drug industry.
这项研究评估了乙醇、甲醇、乙酸乙酯和丙酮提取物对测试的致病真菌（、和）的抗真菌效果。还评估了提取物对四种不同细胞系的抗氧化和细胞毒性效果。甲醇提取物对的抑制效果最好，通过孔扩散法测量。用甲醇提取物处理的细胞的透射电子显微镜显示细胞质细胞器有轻微的溶解和空泡化。在体内，用和甲醇提取物乳膏诱导感染小鼠后，皮肤层出现了去除球形质孔的现象。用 DPPH（2,2-二苯基-1-苦基肼）清除法测定，提取物的抗氧化活性最高（IC 28 毫克/毫升）。用 MTT 法进行的细胞毒性试验表明，提取物对 HepG2 细胞系具有很强的细胞毒性活性（IC 20.56 ± 1.7 μg/mL），对 MCF7 和 Hela 细胞具有中度细胞毒性活性（IC 27.99 ± 2.1 μg/mL）。气相色谱/质谱（GC/MS）结果表明，提取物的有效活性可能与其主要成分生物碱、叶绿醇、脂肪酸烃、酚类和邻苯二甲酸酯之间的协同作用有关。提取物含有活性代谢物，是制药工业中抗真菌、抗氧化和抗增殖化合物的有前途的来源。
