Bohdanovych Taisa, Kuzema Pavlo, Anishchenko Viktor, Duplij Volodymyr, Kharchuk Maksym, Lyzhniuk Viktoriia, Shakhovsky Anatolij, Matvieieva Nadiia
Institute of Cell Biology and Genetic Engineering of NAS of Ukraine, Kyiv, Ukraine, 03143.
Chuiko Institute of Surface Chemistry of NAS of Ukraine, Kyiv, Ukraine, 03164.
Biol Open. 2025 Mar 15;14(3). doi: 10.1242/bio.061739. Epub 2025 Mar 19.
The green synthesis of metal nanoparticles (NPs) has garnered significant attention due to its simplicity, cost-effectiveness, and environmental sustainability. Gold NPs (AuNPs) and silver NPs (AgNPs) are widely employed across various industries, agriculture, and medicine owing to their unique physicochemical properties. This study explores the feasibility of synthesizing metal NPs through green methods using ethanolic (70%) extracts from Artemisia annua hairy roots. These extracts were found to contain reducing agents, primarily phenolic compounds, as identified by HPLC and MALDI-MS analyses. The phenolic compounds included hydroxybenzoic acids (e.g. p-coumaric and gallic acids) and hydroxycinnamic acids (e.g. caffeic acid and its derivatives such as chlorogenic, dicaffeoylquinic, and rosmarinic acids). The synthesis and structural characteristics of AuNPs and AgNPs were systematically compared. AgNPs formed a stable colloidal solution over extended periods, while AuNPs exhibited instability due to significant NP aggregation and precipitation. Furthermore, the photocatalytic activities of these NPs in the degradation of Methylene Blue were evaluated. AuNPs demonstrated substantial photocatalytic activity, whereas AgNPs exhibited negligible catalytic effects. This study highlights the potential and limitations of A. annua hairy root extracts in the biosynthesis of AuNPs and AgNPs, providing insights into their structural and functional differences.
金属纳米颗粒（NPs）的绿色合成因其简单性、成本效益和环境可持续性而备受关注。金纳米颗粒（AuNPs）和银纳米颗粒（AgNPs）由于其独特的物理化学性质而广泛应用于各个行业、农业和医学领域。本研究探讨了使用黄花蒿毛状根的乙醇（70%）提取物通过绿色方法合成金属纳米颗粒的可行性。通过HPLC和MALDI-MS分析发现，这些提取物含有还原剂，主要是酚类化合物。酚类化合物包括羟基苯甲酸（如对香豆酸和没食子酸）和羟基肉桂酸（如咖啡酸及其衍生物，如绿原酸、二咖啡酰奎宁酸和迷迭香酸）。系统地比较了AuNPs和AgNPs的合成及结构特征。AgNPs在较长时间内形成了稳定的胶体溶液，而AuNPs由于显著的纳米颗粒聚集和沉淀而表现出不稳定性。此外，评估了这些纳米颗粒在亚甲基蓝降解中的光催化活性。AuNPs表现出显著的光催化活性，而AgNPs表现出可忽略不计的催化效果。本研究突出了黄花蒿毛状根提取物在AuNPs和AgNPs生物合成中的潜力和局限性，为它们的结构和功能差异提供了见解。
